摘要
目的探讨子宫颈炎组织和子宫颈癌组织中GRP78、CRT、ERP57蛋白与HPV16型感染在维吾尔族和汉族妇女中的表达及相关关系。方法采用免疫组化SP法和PCR技术检测子宫颈炎组织69例(汉族38例,维吾尔族31例)以及子宫颈癌组织124例(汉族36例,维吾尔族88例)中GRP78、CRT、ERP57蛋白的表达和HPV16的感染情况。结果 HPV16型感染在子宫颈癌中的阳性表达率(31.45%)高于宫颈炎组(11.59%),差异有统计学意义(P<0.05)。GRP78、CRT、ERP57 3种蛋白在宫颈癌中的表达(95.16%、90.32%、90.32%)均高于与其各自在宫颈炎中的表达(89.96%、50.72%、49.28%),差异均有统计学意义(P<0.05)。GRP78和CRT在汉族妇女宫颈癌中的表达(100.00%、97.22%)均高于各自在汉族妇女宫颈炎中的表达(81.58%、39.47%),并且差异均有统计学意义(P<0.05)。CRT和ERP57在维吾尔族妇女宫颈癌中的表达(87.50%、94.32%)均高于各自在维吾尔族妇女宫颈炎中的表达(64.51%、35.48%),并且差异均有统计学意义(P<0.05)。除此之外,仅ERP57在维吾尔族妇女宫颈癌中的表达(94.32%)高于其在汉族宫颈癌中的表达(80.56%),差异有统计学意义(P<0.05)。而通过对GRP78、CRT、ERP57蛋白与HPV16的相关性分析发现:GRP78、CRT、ERP57蛋白在维吾尔族妇女宫颈癌中的阳性表达率与HPV16型感染均存在正相关关系(r=0.278,P<0.05;r=0.429,P<0.05;r=0.222,P<0.05)。结论通过对GRP78、CRT、ERP57 3种蛋白和HPV16联合检测,可能作为检查子宫颈癌的标记物,提高宫颈癌的检出率;ERP57蛋白在宫颈癌中的表达存在民族间差异。
Purpose To evaluate the expression levels of the MHC class Ⅰ antigen presentation associated proteins GRP78,CRT and ERP57 and their association with HPV16 infection in human cervical cancer lesions from patients with Uygur and Han ethnicities.Methods The expression of proteins GRP78,CRT and ERP57 in 69 cervicitis tissues including 38 Han and 31 Uygur women and 124 cervical cancer lesions from 36 Han and 88 Uygur women were evaluated with SP immunohistochemistry,and the infection status of HPV in these tissues was studied by PCR using specific primers for HPV16.Results The infection rate of HPV 16 in cervical cancer（31.45%） was higher than that in cervicitis tissues（11.59%） respectively,and the difference was significant（P0.05）.GRP78,CRT and ERP57 in cervical cancer（95.16%,90.32%,90.32%） from both ethnic groups were higher than that in cervicitis tissues（89.96%,50.72%,49.28%）（P0.05）.The expression rates of both GRP78 and CRT in cervicitis from Han women（93.18%,87.50%） were significantly reduced when compared to that in cervical cancer（100.00%,97.22%） from Han women and the differences had also statistical significance（P0.05）.The expression rates of both CRT and ERP57 in cervicitis from Uygur women（64.51%,35.48%） were significantly reduced when compared to that in cervical cancer（87.50%,94.32%） from Uygur women,and the differences had statistical significance yet（P0.05）.In adition,there were significantly differences in expression of ERP57 in cervical cancer tissues between Han（80.56%） and Uygur women（94.32%）（P0.05）.There were positive correlation in expression of CRT,ERP57,GRP78 and HPV in cervical cancers of Uygur women（r=0.429,P0.05;r=0.222,P0.05;r=0.278,P0.05）.Conclusion The detection of GRP78,CRT and ERP57 besides HPV16 may improve diagnosis of cervical cancer.The expressions of ERP57 was significant differences in cervical cancer of ethnic group boths.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2011年第5期463-467,472,共6页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金资助(30460046)
