摘要
目的研究姜黄素对人肾癌ACHN细胞放射的增敏作用,并探讨其作用机制。方法以人肾癌ACHN细胞为研究对象,MTT比色法检测姜黄素对人肾癌ACHN细胞增殖的抑制作用;克隆形成实验检测姜黄素对ACHN细胞的放射增敏作用;流式细胞术检测ACHN细胞周期分布;RT-PCR检测ACHN细胞Bcl-2、Bax、NF-κBP65和DNA-PKcs mRNA的表达。结果姜黄素对人肾癌ACHN细胞生长具有明显的抑制作用,且呈现剂量和时间效应。10μmol/L姜黄素可降低放射后人肾癌ACHN细胞的细胞存活分数(P<0.05),有放射增敏作用,放射增敏比SERDq为2.36。药物+放射组ACHN细胞周期阻滞在放射敏感时相G2/M期。与放射组相比,药物+放射组Bcl-2、NF-κBP65和DNA-PKcs mRNA水平明显降低(P<0.05)。结论姜黄素对人肾癌ACHN细胞具有放射增敏作用,其作用机制可能与其抑制ACHN细胞NF-κB表达,下调Bcl-2/Bax比例,抑制DNA损伤修复,改变ACHN细胞周期分布有关。
Objective To investigate the radiosensitization by curcumin on human renal cell carcinoma cell line ACHN and its possible mechanisms in vitro.Methods The human renal cell carcinoma cell line ACHN was used.MTT assay was used to evaluate the anti-proliferation effect of curcumin on ACHN cells,and clonogenic assay was performed to determine the radiosensitizing effect of curcumin.Flow cytometry was utilized to observe the cycle of ACHN cells.The expression levels of Bcl-2,Bax,NF-κBP65 and DNA-PKcs mRNA were evaluated by RT-PCR method.Results Curcumin could significantly inhibit the proliferation of ACHN cells in the dose-and time-dependent manners.Subtoxic dose of curcumin at 10 μmol/L combined with radiation significantly reduced the survival fraction of ACHN cells（P〈0.05） and enhanced the radiosensitivity of ACHN cells with an SER of 2.36.Curcumin combined with radiation induced cell-cycle redistribution of ACHN cells and prolonged cell cycle G2/M phase arrest.Compared with those in the radiation group,the expression levels of Bcl-2,NF-κBP65 and DNA-PKcs mRNA were decreased significantly in the drug ＋ radiation group（P〈0.05）.Conclusion Curcumin has a radiosensitizing effect on human renal cell carcinoma ACHN cells in vitro.Its mechanisms may be related to its inhibiting NF-κB expression,decreasing the ratio of Bcl-2/Bax,prolonging cell cycle arrest and inhabiting DNA damage repair
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2011年第3期299-302,306,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
姜黄素
肾细胞癌
凋亡
放射增敏
ACHN细胞
curcumin
renal cell carcinoma
apoptosis
radiosensitization
ACHN cell
