摘要
目的：建立一种用短波紫外线（ Ｕ Ｖ Ｃ）与交联淀粉碘（ Ｃ Ｓ Ｉ）联用的血浆消毒实用模型。方法：设计并制作了血浆消毒模型。用辛德比斯病毒（ Ｓ Ｖ）作为试验病毒，通过细胞感染试验测定模型的灭病毒效果。采用免疫化学技术观察被处理血浆中 Ｉｇ Ｇ的活性变化。结果：含病毒血浆首先经剂量为９ ８６０ Ｊ／ｍ ２ 的 Ｕ Ｖ Ｃ照射后，再流经 Ｃ Ｓ Ｉ柱时，血浆在 Ｃ Ｓ Ｉ柱内的滞留时间越长，其病毒的滴度越低，在滞留时间为１２０ ｍ ｉｎ 时，血浆中的病毒灭活达６．７８ 个对数级。而此时，血浆中 Ｉｇ Ｇ的抗原和抗体活性无明显变化。结论： Ｕ Ｖ Ｃ与 Ｃ Ｓ Ｉ联用技术的血浆消毒模型能有效灭活 Ｓ Ｖ 病毒，不损害血浆中抗体成分的活性。
Objective: To construct an effective and practical disinfector for plasma disinfection with short wave ultraviolet rays (UVC) and cross linked starch iodine (CSI). Methods: A disinfector was designed and constructed. Sindbis virus (SV) was used to infect the blood cells. The infected plasma was treated with UVC and CSI. In order to determine the efficacy of disinfection and the viability of the virus, the changes of the IgG activity in the treated plasma was measured with immunochemistry technique. Results: After the infected plasma was irradiated with 9860 J/m 2 UVC, the plasma flowed through a column of CSI. The longer the infected plasma stayed in the CSI column, the lower the titration concentration of SV in the plasma. When the infected plasma stayed in the CSI column for 120 min, the viability of SV was reduced to a degree of log 6.78. Simultaneously, no significant change of the molecular integrity and activity of IgG was found. Conclusion: UVC and CSI could effectively inactivation SV without affecting the antibody activity in the treated plasma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第8期581-584,共4页
Journal of Third Military Medical University
