摘要
目的：研究一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ；ＮＯ）与一氧化氮合酶（ＮｉｔｒｉｃＯｘｉｄｅｓｙｎｔｈａｓｅ；ＮＯＳ）活力对丙氨酸转氨酶（ＡＬＴ）与天冬氨酸转氨酶（ＡＳＴ）活力的影响并探讨其机制。方法：对大鼠腹腔注射Ｌ－Ａｒｇ，４ｈ后采血取肝检测ＮＯ含量，ＮＯＳ活力、ＡＬＴ及ＡＳＴ活力并与对照组大鼠对比．ＮＯ定量用硝酸还原酶法、ＮＯＳ测定用分光光度法、ＡＬＴ与ＡＳＴ测定用改良赖氏法（２，４一二硝基苯肼比色法）。用ＮａＮＯ２和ＮａＮＯ３作抑制剂进行酶抑制试验。结果：腹腔注射Ｌ－Ａｒｇ后肝脏及血清中ＮＯ含量与ＮＯＳ活力显著升高（Ｐ＜０．０１）；而肝脏及血清中ＡＬＴ与ＡＳＴ活力下降。结论：体内ＮＯ转化生成的ＮＯ－２、ＮＯ－３对ＡＬＴ及ＡＳＴ有抑制作用，其中ＮＯ－２的抑制作用强于ＮＯ－３。
Objective:To study the effect on activity of ALT and AST by nitric oxide product and nitric oxide synthase activity Methods:Injecting into abdominal cavity of mice with the injection of 8% L-Arg Four hours later,measuring the nitric oxide product,the activity of nitric oxide synthase and the activity of ALT and AST, and contrasting them with those of the normal subjects NO: nitrate reductase methods NOS: spectrophotometric methods AST and ALT: 2,4 dinitro phenylhydrazine colorimetry The NaNO 2 and NaNO 3 as the inhibitor are used in the inhibitory test on ALT and AST Results:After injecting into abdominal cavity of mouses with the injection of 8% L Arg, the NO product and the activity of NOS in hepar and serum of the mice were increased (P<0 01) Contrary to that, the activity of ALT and AST were decreased (hepar: P<0 01; serum: P<0 05) NO - 2 and NO - 3 are the powerful inhibitors of ALT and AST Conclusion:The NO - 2 and NO - 3 that were inverted by the endogenous nitric oxide are the inhibitors of ALT and AST The inhibition of NO - 2 are more powerful than NO - 3
出处
《广西医科大学学报》
CAS
1999年第5期608-610,共3页
Journal of Guangxi Medical University
关键词
一氧化氮
一氧化氮合酶
ALT
AST
护肝
L Arginine
nitric oxide
nitric oxide synthase
alanine transaminase
asparagine transaminase
