摘要
目的 :探讨广西、海南和云南三地微小按蚊之间的基因差异。方法 :从三地单雌线蚊虫提取 DNA,然后分别用 Ava I、Bam HI、Pst I、Bgl I、Hinf I和 Hae 6种限制性核酸内切酶消化 ,进行琼脂电泳分析。结果 :三地微小按蚊的 Ava I、BamHI和 Pst I酶切区带有差异 ,Bgl I的酶切区带完全相同 ,而 Hinf I和 Hae 两种酶不显区带。结论 :三地微小按蚊有基因结构差异 ,Ava I、 Bam HI和 Pst I
Objective:To observe the variation in repetitive DNA of the An Minimus from Guangxi,Hainan and Yunnan Method:Total DNA was extracted from isofemale lines of the three strains and digested respectively with six restriction endonucleases (Ava I,Bam HI,Pst I,Bgl I,Hinf I and Hae Ⅲ).The DNA fragments were analysed by agarose gel electrophoresis coupled with ethidium bromide staining.The fluorescent DNA bands were visualized under a UV lamp and photographed Results:Banding patterns produced by Ava I,Bam HI and Pst I show some unique DNA fragments Bgl I digestion generally gave similar patterns The latter two enzymes gave vague patterns (results not shown) Conclusion:In view of the variation in repetitive DNA of different area strains of An minimus,Three enzymes(Ava I,Bam HI and Pst I) were capable of distinguishing among the three area strains
出处
《广西医科大学学报》
CAS
1999年第4期405-407,共3页
Journal of Guangxi Medical University
基金
国家自然科学基金!资助项目( 3 946 0 0 74
