摘要
目的:评价核基质结合区结合蛋白与恶性黑素瘤的相关性。方法:以取材自两个健康供者的原代培养黑素细胞和乳癌细胞系MDA-MB-231作为对照,应用RT-PCR方法,检测部分与肿瘤相关的核基质结合区结合蛋白(SMAR1、Lamin B1、Nucleohn、hnRNP U、SATB1、YBX1、CUX1和Lamin 132)mRNA在恶性黑素瘤细胞系A375、M14和MV3的表达。结果:所有被检测核基质结合区结合蛋白在正常黑素细胞均未见表达;在三个恶性黑素瘤细胞系均有表达的有SMAR1、Lamin B1、Nucleolin、hnRNPU、SATB1和YBX1,以Nucleolin、hnRNP U和SATB1的表达水平与正常黑素细胞的差异较大。乳癌细胞系MDA-MB-231与黑素瘤细胞表达谱相似。结论:Nucleohn、hnRNP U和SATB1等MARBP可能参与了黑素瘤的发生与发展。
Objective： To investigate the expression of matrix attachment regions binding proteins （MARBPs） in maliganant melanoma. Method： The expression of ten MARBPs （SMAR1, CUX1, Lamin B1, Nucleolin, hnRNP U, SATB1, YBX1 andLamin B2） in three melanoma cell lines （A375, M14 and MV3） was measured by RT- PCR, with normal primary cultured melanocytes from two healthy donors and breast cancer cell line MDA- MB - 231 as controls. Results： SMAR1, Lamin B1, Nucleolin, hnRNP U, SATB1, and YBX1 were over- expressed in these three melanoma cell lines, especially Nucleolin, hnRNP U, SATB1, similar to MDA - MB - 231, whereas no MARBP was detected in normal melanocytes. Conclusion： These results suggest that some MARBPs, such as Nu- cleolin, hnRNP U and SATB1, may play roles in the development of malignant melanoma.
出处
《中国麻风皮肤病杂志》
2011年第5期307-310,共4页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金(编号:30672474)
江苏省自然科学基金(编号:BK2009064)
高校博士学科点专项科研基金(编号:20091106120001)
关键词
恶性黑素瘤
核基质结合区结合蛋白
表观遗传学
malignant melanoma
matrix attachment regions binding proteins
epigenetics
