摘要
研究了使用Actinobacillus succinogenes菌种以葡萄糖为底物,发酵生产琥珀酸过程中的产物抑制特征,及使用原位分离技术(ISPR)来消除产物抑制现象,以提高底物转化率。研究发现,该菌种的生长与发酵呈现出明显的非偶联特征,在菌体生长进入稳定期(30 h培养)后,开始大量合成产物琥珀酸。当发酵液中琥珀酸浓度高于25 g/L时,开始出现明显的产物抑制作用,底物转化效率逐渐降低。通过模拟发酵液筛选出了对琥珀酸有较强吸附的D301T、D301R、D303树脂,并通过真实发酵筛选出了对发酵体系无毒性的D301R树脂。最后通过5L发酵罐,对树脂吸附原位分离发酵进行验证,在接种后30h将树脂D301R以40 g/L的量添加入5L发酵罐中,至发酵终点琥珀酸产量达到49.46 g/L(底物葡萄糖60 g/L),底物葡萄糖转化率达到82.43%,相比普通发酵过程,底物转化率提高了21%。
The characteristics of product inhibition on the growth of Actinobacillus succinogenes in fermentation using glucose as the major carbon source and the technology of the In Situ Product Removal （ISPR） with resin adsorption to alleviate the product inhibition were studied. The study showed that the characteristic of the cells growing process and the succinic acid synthesizing process was non - coupling, which means that the cells started to synthesize succinic acid in the stationary growth stage （after cultivation for 30 hours）. The product inhibition became more and more obvious when the succinic acid concentration was over 25g/L in the fermentation broth, and the conversion rate of the substrate became decreased. Through simulative fermentation broth, resin of D301T, D301R and D303 have been selected, which have great adsorptive capacity of the succinic acid. D301R was innocuous in the ISPR, which was finally ascertained by using in the real fermentation system. Application effect of the ISPR with resin was confirmed through 5L automatic fermentor, and 40 g/L D301R resin was added after 30 hours cultivation. After the fermentation process was finished, the concentration of the succinic acid can reach 49.46g/L （ the substrate of glucose is 60 g/L） and the glucose conversion rate can reach 82.43% , the substrate conversion rate was increased by 21% comparing with usual fermentation Method.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第4期12-16,共5页
Food and Fermentation Industries
基金
2009年中国科学院理化技术研究所青年基金支持项目
关键词
原位分离
树脂吸附
发酵
底物转化率
In Situ Product Removal （ISPR）, resin, fermentation, substrate conversion rate
