摘要
目的研究改良羊水细胞培养法在产前诊断中的应用。方法采用改良多种处理方式的经典羊水细胞培养法和原位培养法对280例孕16-32周的孕妇进行羊水产前诊断。结果成功率99.7%,培养收获时间5-7天,最短收获5天,最长7天。有效的细胞染色体核型>60个/例,发现异常核型15例,染色体异常检出率5.4%。结论两种改良羊水细胞培养技术,培养成功率高,细胞培养时间短,可供分析染色体核型多,培养适用范围宽,可满足临床产前诊断的需要。
objective：To research application of the reforming of amniotic fluid cells culture in different methods in prenatal diagnosis.Methods： 280 samples of amniotic fluid from pregnant women during 16-32th-week gestation were cultured in the reforming of diverse ways to deal with of amniotic cell culture in classical method of amniotic fluid cell culture and method of situ cultivation.Results：The successful rate was 99.7%.Culture Collection time： 5-7days,the shortest time： 5 days,The most time：7days.Effective karyotypes in each case were more than the 60.Discovered 15 cases of abnormal karyotype.5.4% rate of chromosomal abnormalities detected.Conclusion： The reforming of two amniotic fluid cell culture techniques were superior than the classical method of amniotic fluid cell culture in training success rate,cell culture time,providing the number of analysis of karyotype and training scope.They can meet the needs of clinical prenatal diagnosis.
出处
《中国优生与遗传杂志》
2011年第6期47-48,共2页
Chinese Journal of Birth Health & Heredity
关键词
羊水
改良细胞培养
产前诊断
染色体
核型分析
Amniotic fluid
Reforming of Cells Culture
Prenatal Diagnosis
Chromosome
Karyotype
