摘要
目的:了解广州地区海产品和珠江水中致病性弧菌的污染状况,为卫生行政部门制定相关控制措施提供科学依据。方法:霍乱弧菌按《霍乱诊断标准》WS289-2008附录A和霍乱防治手册(第五版)进行检测,副溶血性弧菌按GB/T4789.7-2008操作,创伤弧菌的检验按国家食源性致病菌监测工作手册中创伤弧菌检验标准操作程序进行。结果:2009年-2010年二年共完成320份样品检测,共检出119株致病菌,检出率为37.19%。其中检出率最高的是副溶血性弧菌,检出率为26.56%,其次为创伤弧菌,检出率为9.06%,O1群霍乱弧菌检出率为1.56%。海产品和珠江水中致病性弧菌污染水平存在季度性差异(P<0.005)。结论:应加强对海产品和珠江水中致病性弧菌的监测,尤其是在温暖季节。
Objective：To learn about the contamination status of pathogenic Vibrio isolated from seafood and Pearl River water in Guangzhou area and to provide scientific data for related health administration in making controlling measures.Methods： Vibrio cholerae were isolated and identified according to Diagnostic criteria for cholera WS 289-2008（Appendix A） and Mannual for cholera prevention and therapy.Vibrio parahaemolyticus were isolated and identified according to National Standards of Microbiological Examination for Vibrio parahaemolyticus（GB/T4789.7-2008）.The isolation and identification of Vibrio vulnificus were performed according to National Surveillance Mannual for food-borne pathogenic bacteria.Results： 119 strains of pathogenic Vibrio were isolated from all 320 samples,with the detection rate being 37.19%.Among them,Vibrio parahaemolyticus had the most highest detection rate（26.56%）,followed by Vibrio vulnificus（9.06%） and Vibrio cholera serogroup O1（1.56%）.Significant differences were observed in the contamination rate among pathogenic Vibrio in different quarter of the year（P0.005）.Conclusion： There is high degree of contamination by pathogenic Vibrio in seafood of Guangzhou area and it was advised by sanitary administrations to take effective measures to prevent food-borne diseases caused by pathogenic Vibrio from happening,especially in warm seasons.
出处
《中国卫生检验杂志》
CAS
2011年第5期1237-1239,共3页
Chinese Journal of Health Laboratory Technology
基金
广州市卫生局医药卫生科技(2006-Zdi-11)
广东省科技计划项目(2008B030303041)
关键词
海产品
珠江水
致病性弧菌
监测
Seafood
Pearl River water
Pathogenic Vibrio
Surveillance
