摘要
目的:研究血管性痴呆(Vascular Dementia,VD)大鼠巨噬细胞-小胶质细胞胞质抗原(ED1)及学习记忆能力的改变。方法:雄性Wistar大鼠30只,随机分为正常对照组、假手术组和VD模型组,采用4-血管阻断(4-Vesselocclusion,4-VO)全脑缺血再灌注制备VD大鼠模型,Y-型迷宫检测各组大鼠学习记忆能力,SABC法检测大脑皮质ED1阳性表达细胞。结果:与正常组、假手术组相比较,模型组大鼠学习、记忆能力受损明显,ED1免疫神经元表达增高(P<0.05)。结论:VD模型大鼠学习记忆能力下降可能与ED1表达增高有关。
Objective： To study the changes of macrophage-microglia antigen（ED1）levels and behavior of vascular dementia（VD） rats,and to explore their relationship.Methods： Thirty male wistar rats were randomly divided into three groups： normal control group（group C）,sham operation group（group S）,VD model group（group M）.VD model was established with 4-vesselocclusion method.Learning and memory abilities of rats in three groups were detected with Y-type maze test.Immunohistochemical method was used to detect the expression of ED1.Results： Compared with those in groups C and S,learning and memory abilities of rats were significantly impaired,and ED1 expression positive neurons were increased（P0.0 5） in group M.Conclusions： The decline of learning and memory abilities of rats may relate to the increasing of ED1 expression in neurons in the cerebral cortex.
出处
《贵阳医学院学报》
CAS
2011年第2期162-164,共3页
Journal of Guiyang Medical College
关键词
痴呆
血管性
大脑皮质
学习记忆能力
免疫组织化学
大鼠
dementia
vascular
cerebral cortex
learning and memory ability
immunohistochemistry
rats
