摘要
目的： 探讨端粒酶活化在喉癌和下咽癌发生发展中的作用。方法： 采用改良的银染端粒重复序列扩增法对５８ 例喉癌下咽癌组织、２５ 例癌旁组织和１０ 例癌前病变组织进行了端粒酶活性检测。结果： ５８ 例喉癌和下咽癌中５３ 例阳性， 阳性率９１４ ％ ； ２５ 例癌旁组织中３ 例阳性， 阳性率１２ ％ ； １０ 例癌前病变中７ 例阳性， 阳性率７０ ％ 。结论： 端粒酶活化在喉癌、下咽癌及其癌前病变组织中表达极高， 对阐明喉癌和下咽癌的发病机理和癌变风险估计有重要意义， 有可能成为咽喉癌早期诊断的生物学标记物。
Objective:To explore the role of telomerase activity in development and progression of laryngeal and hypopharyngeal carcinomas.Methods:A modified telomeric repeat amplification protocol based on PCR technique by silver staining was used to determine telomerase activity in 58 cases of laryngeal and hypopharyngeal carcinomas,in 25 cases of paracancerous tissues and in 10 cases of precancerous lesions.Results:Telomerase activity was detected in 53 of 58(91 4%) cancerous tissues,in 3 of 25(12%) adjacent tissues and in 7 of 10(70%) precancerous lesions.Conclusion:Telomerase activity may play an important role in carcinogenesis of laryngeal and hypopharyngeal carcinomas and in cancer risk assessment with its overexpression,which may be a valuable biomarker for early diagnosis of pharyngeal and laryngeal carcinomas.
出处
《中日友好医院学报》
1999年第4期211-213,共3页
Journal of China-Japan Friendship Hospital
关键词
端粒
激活
喉肿瘤
咽肿瘤
癌前状态
telomere
enzyme activation
laryngeal neoplasms
pharyngeal neoplasms
precancerous conditions
