摘要
建立了化妆品中欧前胡素和异欧前胡素的高效液相色谱-串联质谱(HPLC-MS/MS)测定与确证方法。样品经甲醇提取,以C18柱为分离柱,10 mmol/L乙酸铵/甲醇溶液-5 mmol/L乙酸铵水溶液(80∶20,v/v)为流动相分离,采用电喷雾串联四极杆质谱进行检测。欧前胡素和异欧前胡素在0.25～20μg/L内线性良好(相关系数大于0.999);方法定量限(LOQ)为0.50 mg/kg。在0.50～10.0 mg/kg内,欧前胡素和异欧前胡素的回收率范围分别为82.2%～105%和80.0%～103%,相对标准偏差分别为2.7%～4.9%和1.8%～4.6%。该方法能够满足化妆品中欧前胡素和异欧前胡素检测的需要。
A novel method for the simultaneous determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was developed.The imperatorin and isoimperatorin were extracted by methanol.The analytes were separated on a C18 column using 10 mmol/L ammonium acetate/methanol-5 mmol/L ammonium acetate aqueous solution（80∶20,v/v） as mobile phase,and then detected by HPLC-MS/MS using multiple reaction monitoring（MRM） mode.The good linearities（r0.999） were achieved for the target compounds over the range of 0.25-20 μg/L.The limits of quantification（LOQs） were 0.5 mg/kg.The recoveries for the imperatorin and isoimperatorin varied from 82.2% to 105% and 80.0% to 103% with the relative standard deviations ranging from 2.7% to 4.9% and 1.8% to 4.6% respectively in the range of 0.50-10.0 mg/kg.The method can meet the requirements for the simultaneous determination of imperatorin and isoimperatorin in cosmetics.
出处
《色谱》
CAS
CSCD
北大核心
2011年第5期454-457,共4页
Chinese Journal of Chromatography
基金
检验检疫行业标准制定计划(No.2009B093)
关键词
高效液相色谱-串联质谱
欧前胡素
异欧前胡素
化妆品
high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）
imperatorin
isoimperatorin
cosmetics
