摘要
以白蓝茎尖、茎段、叶柄、叶片为外植体材料,在培养基MS+6-BA 2 mg/L+NAA 0.2 mg/L上诱导培养。茎尖、茎段、叶片和叶柄愈伤组织诱导率分别为52.4%,76.9%,21.5%和19.3%。在培养基MS+6-BA 0.2 mg/L+NAA 0.1 mg/L上继代培养,茎尖、茎段、叶柄和叶片形成的愈伤组织芽增殖倍数分别为3.9,4.6,3.5和3.1。在培养基1/2 MS+IBA0.1mg/L上生根培养,生根率均在95%以上。
The bud,stem-segment,leafstalk and leaf of Brassica pekinenis × B.olercea as the explants were cultured in tubes containing MS medium supplemented with 2.0 mg·L-1 6-BA and 0.2 mg·L-1 NAA.The bud,stem-segment,leafstalk and leaf could induce callus.The percentage of callus formation were 52.4%,76.9%,21.5% and 19.3%.On MS medium containing 0.2 mg·L-1 6-BA and 0.1 mg·L-1 NAA,shoot multiplication occurred at 3.9,4.6,3.5 and 3.1-fold.Over 95% rooted on 1/2MS medium containing 0.1 mg·L-1 IBA.
出处
《长江蔬菜》
2011年第10期23-24,共2页
Journal of Changjiang Vegetables
关键词
白蓝
组织培养
再生能力
效应
Brassica pekinenis × B.olercea
Tissue culture
Regeneration ability
