摘要
以鱼、虾、贝的可食部分作为试验基质,建立了水产品中顺式氯丹、反式氯丹和氧氯丹的气相色谱测定方法。取均质试样1 g,加乙腈超声提取,正己烷反萃,弗罗里硅土-硅胶层析柱净化,使用配有电子捕获检测器的气相色谱仪测定氯丹含量。氯丹质量浓度线性范围为0.200~100μg.L-1（r=0.998 3~0.999 9,n=7,P〈0.001）,3种氯丹的定量检测下限均为2.00μg.kg-1（试验基质质量以湿质量计）。当加标水平为1~15倍定量限时,6次重复测定的加标回收率为72.2%~104.0%,批次内变异系数为2.81%~8.56%（n=6）,批次间变异系数为8.50%（n=3）。
To establish a method for determination of cis-chlordane,trans-chlordane and oxychlordane in aquatic products with gas chromatography,edible parts of fish,shrimps and shells were used for the test.One gram of homogenized tissues was treated with acetonitrile,extracted ultrasonically,stripped with hexane,and refined through a Florisil silica-gel column.And the outflow from the column was analyzed on a gas chromatograph equipped with electron capture detector for determination of chlordane.The linear range of chlordane concentration was from 0.200 to 100 g·L-1（r = 0.998 3~0.999 9,n=7,P0.001）,and the detection limit for the three species of chlordane was all 2.00 μg·kg-1（in wet weight）.When the sample was added at a rate of 1 to 15 times as much as the quota,the recovery rate of the sample ranged from 72.2% to 104.0% based on 6 repeated determinations.Variation coefficient varied from 2.81% to 8.56% in each batch（n=6） and was 8.50% between batches（n=3）.
出处
《生态与农村环境学报》
CAS
CSSCI
CSCD
北大核心
2011年第3期98-102,共5页
Journal of Ecology and Rural Environment
基金
2010年农业行业标准制定项目(2010-311)
关键词
水产品
鱼
虾
贝
农药
氯丹
检测
气相色谱法
aquatic product
fish
shrimp
shell
pesticide
chlordane
determination
gas chromatography
