摘要
目的了解脊柱关节病（SpA）患者骶髂关节中结缔组织生长因子（CTGF）、Ⅰ型胶原、Ⅲ型胶原的表达情况，探讨CTGF在SpA关节软骨纤维化、骨化、关节强直中的作用。方法30例sDA患者（17例双侧影像学骶髂关节炎≥Ⅱ级，13例影像学骶髂关节炎Ⅰ级）均接受CT引导下骶髂关节穿刺活检术，取得骶髂关节组织。组织标本均行苏木素一伊红（HE）染色确认存在骶髂关节炎后，通过免疫组织化学染色方法，标记CTGF、Ⅰ型胶原及Ⅲ型胶原的表达情况。统计学方法采用单因素方差分析和t检验。结果30例SpA患者骶髂关节组织中CTGF主要在血管翳炎症细胞及骨髓细胞的胞质中高度表达，阳性细胞数明显多于正常组织对照组[（57．9±42．4），高倍视野和（2．7±2．5），高倍视野）；Ⅰ型胶原及Ⅲ型胶原明显沉积于骨、部分软骨及韧带，平均吸光度均明显高于对照组（分别为0．298±0．080和0．044±0．024；28．254±41．165和0．105±0．054）。结论SpA骶髂关节中存在CTGF的高表达，Ⅰ型胶原及Ⅲ型胶原的沉积增多，提示CTGF参与了SpA骶髂关节局部胶原沉积、软骨纤维化变性过程，可能在SpA关节软骨纤维化、关节强直中起重要的作用。
Objective To investigate the expression of connective tissue growth factor （CTGF）, collagen Ⅰand collagen Ⅲin sacroiliac joint （SIJ） of patients with spondyloarthropathy （SPA）. Methods Thirty patients with SpA, including 17 patients with grade Ⅱsacroiliitis and 13 patients with grade Ⅰsacroiliitis, were performed on CT guided needle biopsy of SIJ. After sacroiliitis were confirmed by staining with hematoxylin and eosin in sacroiliac joint tissue sample, immunohistochemical assay was performed to determine the expression of CTGF, collagen Ⅰand collagen Ⅲ in sacroiliac joint tissue. Univariate Chi-square test was used for data comparison between multiple groups and t-test was used for two group data comparison. Results Contrast to healthy controls, CTGF were found upexpressed on the cytoplasm of inflammatory cells in pannus and bone marrow of sacroiliac tissue samples of patients with SpA, while collagen Ⅰand collagen Ⅲwere found up-expressed in bone, cartilage and ligament tissue [ （57.9＋42.4）/HP vs （2.7＋2.5）/HP P〈0.05, 0.298_＋ 0.080 vs 0.044_＋0.024 and 28.254_＋41.165 vs 0.105_＋0.054, P〈0.05 respectively]. Conclusion CTGF, collagen Ⅰand collagen Ⅲare up-expressed in SIJ of SpA patients. CTGF may play an important role in articular cartilage fibrosis and ossification of SpA.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2011年第5期329-331,361,共4页
Chinese Journal of Rheumatology
基金
广东省科技计划项目（20098030801071）
广东省医学科研基金（A2009619）
深圳市卫生局科技项目（200803012）
关键词
生长物质
胶原Ⅰ型
胶原Ⅲ型
脊柱关节病
骶髂关节
Crowth substances
Collagen type Ⅰ
Collagen type m
Spondyloarthropathy
Sacro-iliac joint
