摘要
目的探讨程序性细胞凋亡基因一1（PD-1）单核苷酸多态性（SNP）与IFN-α治疗慢性乙型肝炎（CHB）患者发生早期病毒学应答的关系。方法采用前瞻性队列研究方法，应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析技术，检测135例中国汉族抗病毒初治CHB患者的PD-1．1和PD-1．2的SNP，并分析其与IFN-α早期病毒学应答的关系。分类变量资料采用γ。检验。结果135例CHB患者IFN-α治疗获得早期病毒学应答有33例，占24．4％。PD-1．1的AA、AG、GG基因型各占35、77和23例，IFN—α的早期病毒学应答各有5、25和3例，分别占14．3％、32．5％和13．0％（x^2=6．258，P=0．044），AG基因型相对AA、GG基因型有较高的病毒学应答率（x^2=6．246，P=0．012）。在PD-1．2的AA、AG、GG基因型之间比较IFN-α的早期病毒学应答，差异无统计学意义（x^2=3．957，P=0．138）。结论PD-1．1的SNP与中国汉族CHB患者IFN-α治疗早期病毒学应答有关。
Objective To investigate the relationship between the single nucleotide polymorphisms （SNP） of programmed cell death-1 （PD-1） gene and early virologic response of interferon-a （IFN-α） in patients with chronic hepatitis B （CHB）. Methods A total of 135 CHB patients were prospectively enrolled in this study. SNP of PD-l. 1 and PD-1.2 genes were examined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in these patients. Then its relationship with early virologic response to IFN-α treatment was analyzed. The data were analyzed by 2 test. Results Among the 135 patients, 33 （24.40/00） achieved early virologic response to IFN a. There were 35, 77, and 23 patients with AA, AG, GG genotgpe of PD-1.1. The early virologic response was achived in 5（14.3%）, 25（32.5%） and 3（13.0%） among patients with AA, AG, GG genotypes of PD-1.1, respectively. There were statistically different （x^2 = 6. 258, P = 0. 044）. The subjects with AG genotype showed higher response rate than those with AA or GG genotypes （X^2 = 6. 246, P= 0. 012）. However, the early virologic response rates were not significant different among subjects with AA, AG or GG genotype of PD-1. 2 （X^2- 3. 957, P = 0. 138）. Conclusion SNP of PD-1. 1 gene may be used as a marker to predict the early virologic response to IFN-α treatment in Chinese CHB patients.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2011年第4期221-225,共5页
Chinese Journal of Infectious Diseases
基金
国家“973”高技术研究发展计划前期研究专项课题基金资助项目（2009CB526411）
国家自然科学基金资助项目（30771907）
高等学校博士学科点专项科研基金资助项目（2∞93420120005）
关键词
肝炎
乙型
慢性
细胞凋亡基因
多态性
单核苷酸
干扰素Α
抗体生成
汉族
Hepatitis B, chronic
Cell apoptosis genes
Polymorphism single nucleotide
Interferon-alpha
Antibody formation
Han nationality
