摘要
用Sephadex G-75凝胶层析技术对亚急性镉中毒肾损害大鼠肝、肾、血、尿中金属硫蛋白(MT)进行了分离测定。结果表明,大鼠接受镉后,其肝、肾、血、尿中MT增多;MT是体内镉的主要存在形式;尿中MT增多是镉中毒肾损害最早出现的变化之一,是肾小管功能障碍的灵敏指标,对反映镉性肾损害有其特异性。
Male Wistar rats were treated with CdCl2 ( 0.592mgCa++/kg, ip., 5 times a week up to 9 weeks). Metallothionein(MT ) in the urine, blood and supernatants of liver and renal cortex ho mogenates were isolated by Sephadex G-75 column chromatography and measured by UV-AAS. MT levels in them were increased after cadmium exposure. Most of the cadmium were bound to MT. These suggest that MT may play a major role in transfer, accnmulation and excretionof cadmium in the body. Increase of MT in urine was not only the earliest change as a low molecular weight protein, but also the specific index of Cd-induced renal damage as a Cd-binding protein.
出处
《卫生毒理学杂志》
CSCD
1990年第3期135-138,194,共5页
Journal of Health Toxicology
基金
国家自然科学基金
关键词
镉中毒
肾损害
金属硫蛋白
尿
Cadmium toxicity
Renal damage
Metallothionein
( MT )
Urine
Rat
