摘要
目的:研究老鼠簕生物碱A(IA)及其衍生物乙酰老鼠簕生物碱A(ACO-IA)的抗炎镇痛作用及对小鼠的急性毒性、胃肠刺激作用。方法:采用改良寇氏法计算小鼠LD50并观察毒性作用,采用二甲苯致小鼠耳肿胀法、小鼠热板法、醋酸扭体法观察其抗炎镇痛作用,并观察连续灌胃给药对小鼠胃肠的影响。结果:IA的对小鼠的半数致死量即LD50值为2 198.87 mg.kg-1,ACO-IA对小鼠的LD50值为2 009.43 mg·kg-1。IA和ACO-IA灌胃给药对冰醋酸所引起的小鼠炎性疼痛有显著抑制作用,能够减轻二甲苯所致的小鼠耳肿胀程度,且作用与阿司匹林相当,而对小鼠胃刺激性比阿司匹林小。结论:按毒性分级标准2个化合物属于低毒性的药物,且均具有抗炎镇痛作用,能够减轻小鼠胃黏膜的损伤程度。
OBJECTIVE To evaluate the anti-inflammatory and analgesic effects,acute toxicity and the gastrointestinal irritation of ilicifolius alkaloids A（IA） and its derivatives acetyl ilicifolius alkaloids A（AcO-IA） in mice. METHODS The anti inflammatory effect was observed in auricle swelling test induced by xylene in mice; analgesic effect was observed by the change of pain threshold in hot-plate test, and the acetic acid-induced writhing test in mice. The modified Karber method was used to determine LD50 of ilicifolius alkaloids A and its derivatives acetyl ilicifolius alkaloids A; the gastrointestinal irritation was observed in mice by continuous intragastric administration. RESULTS IA and AcO-IA could significantly inhibit inflammatory pain in mice that caused by the acetic acid, and evidently suppressing the auricle edema induced by dimethylbenzene in mice. Both effects had no significant differences compared with aspirin, but had less gastrointestinal lesions than that of aspirin. The LD50 of IA in mice was 2 198.87 mg· kg-1 , and LD50 of Ac O-IA in mice was 2 009. 43 mg· kg-1. CONCLUSION According to the grading standard of toxicity, the two compounds belong to the low toxicity of drugs. They have analgesic and anti inflammatory effects, and are able to reduce gastric mucosal damage in mice.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第10期807-810,共4页
Chinese Journal of Hospital Pharmacy
基金
广西科学基金资助项目(编号:0342003-4)
关键词
老鼠筋生物碱A
乙酰老鼠筋生物碱A
半数致死量
抗炎
镇痛
胃刺激
ilicifolius alkaloids A
acetyl ilicifolius alkaloids A
LD50
anti-inflammatory
analgesic
gastric stimulation
