摘要
目的了解宫颈癌患者外周血、肿瘤组织中Th17／Tr细胞的比例，分析其与疾病发生、发展的关系，探讨Th17／Tr比值改变在宫颈癌临床检测中的意义。方法采用流式细胞术检测32例宫颈癌患者外周血、肿瘤组织标本和15例健康者对照标本中的T17、Tr细胞；采用直线相关检验方法对晚期宫颈癌组的Th17、Tr细胞进行相关性分析。结果宫颈癌患者外周血、肿瘤组织中Th17、Tr细胞显著高于健康对照组（P〈0．05），晚期宫颈癌组Th17／Tr比值低于早期组（P〈0．01），晚期宫颈癌组T17、Tr表达呈负相关。结论宫颈癌患者T17、Tr细胞比例明显升高，提示Th17／Tr比值与宫颈癌的发生发展可能存在着一定的关系，为宫颈癌的免疫治疗提供新思路。
Objective To detect the levels of Thl7 and regulatory T（Tr） cells in peripheral blood mononuclear and tumor tissue from patients with cervical cancer and analyze the relationship between Th17 and Tr cells in cervical cancer progression. In addition, the significance of the Th17/Tr cells ratio in cervi- cal cancer pathogenesis was discussed. Methods The expression levels of Th17 and Tr cells were determined by flow cytometry from 32 patients with cervical cancer and 15 health people. The mechanism of in- volvement of Thl7 and Tr cells proportionality in cervical cancer pathogenesis and the correlation between Thl7 and Tr was assessed by bivariate correlation analysis. Results The expression levels of both Th17 and Tr in patients were higher than control groups, especially in late stage patients Th17 and Tr proportionality lower than early group, and there was a negative correlation between them. Conclusion Thl7 and Tr cells proportionality may be involve in the development of cervical cancer so as to provide novel strategies for tumor immunotherapy.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2011年第4期312-315,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助项目（30872335）
镇江市科技计划项目（SH2005047）
