摘要
A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3＋ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3＋ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3＋ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3＋ had no effect on the transdifferentiation of OBs at tested concentrations. Er3＋ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10–6 mol/L Er3＋. These findings suggested that Er3＋ might have negative effect on bone metabolism.
A series of experimental methods including 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H tetrazolium bromide （MTT） test,alkaline phosphatase （ALP） activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR （qRT-PCR） were employed to assess the effects of Er3＋ on the proliferation,differentiation and mineralization function of primary osteoblasts （OBs） in vitro at cell and molecular levels. The results indicated that Er3＋ inhibited the proliferation of OBs at a concentration of 1×10–7 mol/L,but had no effect at other concentrations. Er3＋ inhibited the differentiation of OBs at concentrations of 1×10–8,1×10–7,and 1×10–6 mol/L,but had no effect at a higher concentration of 1×10–5 mol/L. Er3＋ had no effect on the transdifferentiation of OBs at tested concentrations. Er3＋ inhibited the mineralization function of OBs at concentrations of 1×10–7,1×10–6,and 1×10–5 mol/L,but had no effect at a lower concentration of 1×10–8 mol/L. The expression of the mRNA for runt-related transcription factor 2 （RUNX-2） and peroxisome proliferators activated receptor γ （PPAR-γ） was down-regulated in the presence of 1×10–6 mol/L Er3＋. These findings suggested that Er3＋ might have negative effect on bone metabolism.
基金
Project supported by the National Natural Science Foundation of China (20971034)
Foundation for Key Program of Ministry of Education of China (208018)
Returned Scholars of Hebei Province (207041)
Nature Science Key Foundation of Hebei Province (B2009000161)
Natural Science Foundation of Hebei University
