摘要
目的探讨PSAmRNA与f-PSA/t-PSA联合检测在前列腺癌早期诊断中的应用价值。方法 RT-PCR检测前列腺癌(PCa)、良性前列腺增生(BPH)患者及健康对照者外周血中PSAmRNA的水平,应用ELISA方法检测患者血清中的f-PSA及t-PSA浓度。结果 40例PCa标本中PSAmRNA阳性率(80%)及f-PSA/t-PSA比值与正常对照组比较均具有显著性差异(P<0.05)。f-PSA/t-PSA比值<0.15诊断的敏感度最高(87.5%),但是其特异度只有72%,联合检测PSAmRNA和f-PSA/t-PSA比值将特异性提高至97.7%。结论联合检测外周血PSA、PSAmRNA是一种早期发现PCa,判断其临床变化过程、分期、预计复发和评价疗效的方法。
Objective To evaluate the expression of PSAmRNA and free/total prostate-specific antigen （f/t-PSA） in patients of prostatic carcinoma （Pca）,and approach the importance on detecting the PSAmRNA and f-PSA/t-PSA in prostatic carcinoma patients.Methods RT-PCR was used to detect the level of PSAmRNA from PBMC;The PSA and f-PSA was detected by ELISA methods.Results The radio of PSAmRNA positive in peripherial blood in Pca group show significant higher than control group（P〈0.05）,while the f-PSA/t-PSA show significant lower than control group with contral group.The sensitivity of f-PSA/t-PSA0.15 is highest （87.5%）,but specificity lower （72.0%）;The sensitivity combining f-PSA/t-PSA and PSAmRNA was lowest （72.5%）,but the specificity was highest （97.7%）.Conclusion Conclusion PSAmRNA and Serum f-PSA/t-PSA could be useful markers for diagnosing prostate cancer.Combining the PSAmRNA with f-PSA/t-PSA can increase specificity of diagnosising the prostatic carcinoma.
出处
《当代医学》
2011年第15期5-7,共3页
Contemporary Medicine
关键词
前列腺癌
PSA
PSAmRNA
F-PSA/T-PSA
Prostatic carcinoma
Prostate-specific antigen （PSA）
PSAmRNA
free/total prostate-specific antigen ration （f/t-PSA）
