摘要
目的研究毒蕈碱受体在豚鼠输尿管Cajal间质细胞（ICC）上的表达,初步探讨ICC在胆碱能神经递质传递中的作用。方法将20只豚鼠随机分成对照组和实验组,进行输尿管肌条实验,观察在卡巴胆碱作用下输尿管肌条的收缩幅度和频率的变化;10只豚鼠,将输尿管进行固定、撕片和荧光染色,检测豚鼠输尿管毒蕈碱受体亚型（M1-M5）在ICC上的表达情况。结果卡巴胆碱作用后,实验组肌条的收缩幅度为0 g,频率为0次/分,对照组的幅度为（0.106±0.021）g,频率为（4.900±0.875）次/分,两组差异显著（P〈0.01）。免疫荧光双标显示,输尿管ICC仅表达M2、M3受体亚型。结论胆碱能神经可能通过ICC来调控输尿管平滑肌。
Objective To study the expression of muscarinic receptors in interstitial cells of Cajal（ICC） and to discuss preliminarily on the role of ICC in the transmission of cholinergic neurotransmitter.Methods Twenty guinea pigs were randomly divided into control and experiment group.The changes of ureteral muscle contraction amplitude and frequency were observed after applying ureteral muscle strip experiment in vitro.Fixation,tear film and fluorescence staining were performed on the ureters of 10 guinea pigs.The expressions of the muscarinic receptors subtypes（M1～M5） in ICC of guinea-pig ureters were detected.Results Statistical differences were observed between the two group in ureteral muscle contraction amplitude and frequency,which were（0） g and（0） per minute in experiment group and（0.106±0.021） g and（4.900±0.875） per minute in control group（P〈0.01）.Immunofluorescence double labeling showed that ureteral ICC only expressed M2 and M3 receptor subtypes.Conclusion Cholinergic nerve may regulate ureteral smooth muscle by ICC.
出处
《局解手术学杂志》
2011年第2期120-123,共4页
Journal of Regional Anatomy and Operative Surgery
基金
国家杰出青年基金项目(39925022)
