摘要
The synthetic route of N^2, 3-ethenoguanosine （ε-G） was described. Its ultraviolet and fluorescent properties at various pH were studied. These results indicate that ε-G dissociates a hydrogen ion to give the anion ε-G and associates a hydrogen ion to give a the cation ε-GH~. The neutral specie ε-G is the origin of fluorescence. The fluorescent properties in different media were studied and there was most strong fluorescence in water. Based on fluorescencc quenching of ε-G on BSA, the binding constant and the number of binding sites between ε-G and BSA were determined, which were 1.78x10^3 L/mol and I, respectively. The quenching mechanism was discussed. It indicates that the powerful interaction exists between ε-G and BSA.
