摘要
目的：研究原发性肝细胞癌（ＨＣＣ）５ 对染色体３８ 个位点等位基因杂合性丢失（ＬＯＨ） ，ＬＯＨ 与肝癌的临床病理改变和肝炎病毒感染的关系。方法：用ＰＣＲ 微卫星多态性分析检测肝癌ＬＯＨ。结果：发生高频率ＬＯＨ 的位点有染色体１ｐ 的Ｄ１Ｓ１８６（ｐ３１ ， ４８－１％ ） 和Ｄ１Ｓ２４３（１ｐ３６－３ ， ５１－６ ％ ） ； 染色体９ｐ２４ 的Ｄ９Ｓ５４（６１－８％ ） 、９ｐ２１ 的Ｄ９Ｓ１７４７（５２－４％ ） 和ＤＤ９Ｓ１７５２（５１－８ ％ ） 位点；１０ 号染色体１０ｑ２３ ～２４ 区Ｄ１０Ｓ１２２３（５３－３ ％ ） 和Ｄ１０Ｓ１２２５（３８－７ ％ ）位点； １６ 号染色体１６ｑ２４ 区的Ｄ１６Ｓ４１３ 位点ＬＯＨ 频率最高（７０－８ ％ ） ； １７ 号染色体１７ｐ１３ 上ＴＰ５３（５３－８ ％ ） 和Ｄ１７Ｓ５２０（５６－８％ ） 。各染色体ＬＯＨ 与肝癌的临床病理改变无明显关系；ＨＣＶ 和ＨＢＶ 复合感染与ｐ５３ 和ｐ１５ＩＮＫ４Ｂ基因的ＬＯＨ 有关。结论： 肝癌的发生、发展与多染色体的ＬＯＨ 有关， 在染色体１ｐ３１ ， １ｐ３６～ｐ３５ ， ９ｐ２４ 和９ｐ２１ ，１０ｑ２２ ～２４ ，１６ｑ２４ 和１７ｐ 可能存在多个与ＨＣＣ 有关的ＴＳＧ。
Objectives:Study of loss of heterozygosity (LOH) at 38 loci on five chromosomes in 38 cases of hepatocellular carcinoma,and the possible relations betwee the LOH and clinicopathologic changes and hepato viral infection of the HCC.Methods:PCR based microsatellite polymorphism analysis technique was used to detect LOH in HCC.Results:High frequency of LOH was detected on chromosome 1p36 concentrated at locus D1S243 (51 6%)and 1p31 at locus D1S186 (48 1%). On 9p24 at locus D9S54 (61 8%) and 9p21 concentrated at loci D9S1747 (52 4%) and D9S1752 (51 8%),on chromosome 10, only locus D10S1223 (53 3%)on 10q23～24 showed high frequency of LOH. All 5 loci detected on chromosome 16 showed high frequency of LOH more than 50%,with the highest frequency of LOH at locus D16S413 (70 8%) on 16q 24. On chromosome 17, high frequency of LOH concentrated on 17p13 at TP53 gene locus (53 8%)and locus D17S520 (56 8%). HBV and HCV coinfection in HCC is associated with LOH at TP53 locus and p15 gene locus.Conclusions:The results indicated that high frequency of LOH on multiple chromosome arms were involved in hepatocarcinogenesis,there may be more than one putative TSG harbored in these chromosome arms associated with HCC.Coinfection of HBV and HCV is closely associated with LOH at p53 and p15 gene loci.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第5期520-523,共4页
Chinese Journal of Cancer
基金
香港政府科学研究基金!资助
基金号:CU95661/UPG
关键词
肝细胞癌
杂合性丢失
染色体
抑制基因
Hepatocellular carcinoma
Loss of heterozygosity
Tumor suppressor gene
Chromosome
