摘要
目的观察在不同浓度的雌激素作用下,卵巢癌细胞转化生长因子-β1（TGF-β1）、血管内皮细胞生长因子C（VEGF-C）表达的变化。方法在体外不同浓度（10-11、10-10、10-9、10-8mol/L 17β-E2）的17-β雌二醇（E2）作用下,用半定量逆转录聚合酶链反应（RT-PCR）及ELISA方法,检测卵巢癌SKOV3细胞株VEGF-CmRNA的表达及TGF-β1分泌量水平的变化。结果 SKOV3细胞在E2的作用下,TGF-β1的分泌量呈剂量浓度反应,随着10-11~10-8mol/L17-βE2浓度的增加,TGF-β1的分泌量明显增加;随着雌激素浓度的增加,SKOV3细胞VEGF-C mRNA的PCR产物表达也呈递增趋势。10-11、10-10、10-9和10-8mol/L 17β-E2组与对照组比较,VEGF-C,TGF-β1的表达有显著性差异（P〈0.05）。结论雌激素可上调SKOV3细胞的VEGF-C mRNA表达、TGF-β1分泌水平,两者均呈浓度递增趋势。
Objective To investigate the effect of 17β-estradiol（17β-E2） on expression of VEGF-C mRNA and transforming growth factor-β1（TGF-β1） in epithelial ovarian carcinoma cell line in vitro.Methods Human epithelial ovarian carcinoma cell line SKOV3 cells were exposed to 17β-2 E2（10-11~10-8 mol/ L）.The expression level of VEGF-CmRNA was determined by RT-PCR.TGF-β1 was investigated by using ELISA.Results The expression of VEGF-CmRNA and TGF-β1 in the 17β-E2 treated cells was significantly higher than in the untreated control（P0.05）,the expression level of VEGF-CmRNA and TGF-β1increased with the increase in 17β-E2 concentration.Conclusion Estrogen can up-regulate expression of VEGF-CmRNA and TGF-β1.Further studies are needed to elucidate the mechanism.
出处
《西部医学》
2011年第5期825-827,共3页
Medical Journal of West China
