摘要
目的采用微板核酸杂交-ELISA技术测定肝炎病人血清中HBVDNA含量。方法用PCR法将病人血清标本扩增后，与两条特异性探针夹心杂交，然后通过酶联显色对69例不同临床表现的肝炎患者血清中HBVDNA进行定量检测。结果20例急性重型肝炎血清中的DNA含量超过2μg/L，88.24％的急性轻型肝炎患者和54.55％轻度慢性肝炎患者血清中的HBVDNA含量少于2μg/L,66.67％中度慢性肝炎患者血清HBVDNA含量在2-1000μg/L的范围，75％重度慢性肝炎和45％急性重型肝炎血清中的HBVDNA含量超过1000μg／L。结论做饭核酸杂交-ELISA检测方法能准确、快速进行核酸定量测定，特异性强，灵敏度高，稳定可靠，易自动化，对于研究病毒感染量与临床病程的关系具有较大的现实意义。
Objective To quantify HBV DNA in hetatitis patient serum using sandwich hybridization on microplate. Methods HBVDNA extracted from 69 hepatitis patients serum by PCR was hybridized to two specific probes, one of which one was capture probeand the other was detector probe labeled with 5'-biotin to quantitatively assay HBV DNA in hepatitis patient serum. Results SerumLIBV DNA level was more than 2 μ g /L serum in 20(28.98%) heavy acute hepatitis patients, and ess than 2μ g/L in 88.24% lightacute hepatitis patients and in 54.55% light chronic hepatitis patients and between 2 μ g/L and 1 000μ g/L in 66.67% middlechronic hepatitis patients and in 75% heavy chronic hepatitis patients. Coriclusions Sandwich hybridization-ELISA on micropiateis a more sensitive and specific clinic method for detecting quantitatively HBV DNA in hepatitis patient serum.
出处
《第一军医大学学报》
CSCD
1999年第4期354-356,共3页
Journal of First Military Medical University
