摘要
目的研究鹰嘴豆芽乙醇提取物(CSE)对人结肠腺癌Caco-2细胞增殖及细胞凋亡的影响。方法提取鹰嘴豆芽的活性成分并测定含量;MTT法观察提取物对Caco-2细胞增殖的影响;透射电镜观察细胞超微结构;琼脂糖凝胶电泳法进行细胞凋亡的DNA分析;流式细胞术检测凋亡情况。结果 CSE含23.53%皂苷、14.73%异黄酮;不同浓度的CSE对Caco-2细胞均有一定的增殖抑制作用,且呈量效和时效关系;细胞出现染色质边移和凋亡小体;DNA电泳呈现梯度条带,表明CSE可诱导Caco-2细胞凋亡;3、5、10μg/ml的CSE作用后,细胞凋亡率分别为32.6%、68.8%、73.9%,与对照组(0.9%)比较差异显著(P<0.05)。结论 CSE可通过诱导细胞凋亡而抑制人结肠腺癌株Caco-2细胞的生长,为鹰嘴豆应用于结肠癌的临床治疗和预防提供了理论依据。
Objective To explore the effects of chickpea sprout ethanol extract（CSE）on the proliferation and apoptosis of human colon adenocarcinoma cell line Caco-2 in vitro.Methods The active elements of chickpea sprout-CSE,were extracted and its components were analyzed.The proliferation inhibition of CSE on Caco-2 cells was measured by MTT assay.The ultrastructure of these cells was observed under electron microscope.Apoptosis was assayed by agarose gel electrophoresis,and cell apoptosis rate was evaluated by flow cytometry.Results CSE contained 23.53% of saponins and 14.73% of isoflavones.It inhibited Caco-2 cell proliferation in a dose-and time-dependent manner.Caco-2 cells treated with CSE showed chromatin margination and a few apoptotic bodies under electron microscope.DNA electrophoresis displayed a gradient band,which indicated that CSE could induce the apoptosis of Caco-2 cells.After treatment with CSE of 3,5,10 μg/ml for 24 h,the apoptosis was 32.6%,68.8% and 73.9%,respectively（P0.05）.Conclusion CSE can significantly inhibit the proliferation of Caco-2 cells by inducing the apoptosis,which provides the rationale for the application of chickpea to prevention and cure of human colon adenocarcinoma.
出处
《西南国防医药》
CAS
2011年第5期469-472,共4页
Medical Journal of National Defending Forces in Southwest China
基金
中国科学院
国家外国专家局创新团队国际合作伙伴计划
"973"计划前期研究专项(2009CB526509)
新疆维吾尔自治区科技支疆项目(201091242)
