摘要
目的通过对大鼠总IgE、特异性IgE及IgE介导的肥大细胞脱颗粒动态观察,进一步探讨肥大细胞介导的ADCC效应在旋毛虫病免疫中的作用。方法采用雄性Wistar大鼠为旋毛虫感染的动物模型,将90只大鼠随机分为10组,以ELISA双抗体夹心法和间接法动态检测总IgE、特异性IgE,以直接法进行肥大细胞脱颗粒试验,然后采用体外CO2培养法观察免疫血清存在下肥大细胞杀伤旋毛虫肌幼虫作用。结果在免疫血清存在时,感染鼠和正常鼠肥大细胞对旋毛虫幼虫均有杀伤作用,48 h时杀虫数分别为31.5和17.5条(χ2=13.146,P<0.01);χ2=28.543,P<0.01),以感染鼠肥大细胞杀伤作用更强。结论在IgE类抗体依赖肥大细胞介导杀伤旋毛虫肌幼虫的ADCC中,肥大细胞的作用较强。
Objective Determining various indices like total IgE and specific IgE to further investigate the role of ADCC activity triggered by mast cells as part of the immune response to muscle larvae in rats with trichinosis. Methods Male Wistar rats served as an animal model of Trichinella spiralis infection. Ninety rats were divided into 10 groups at random, with 9 rats in each group. Total IgE and specific IgE were detected using double-antibody sandwich enzyme-linked immunosorbent assay （ELISA） and indirect ELISA, and then mast cells with the highest level of IgE were selected and cultured in vitro in a CO2 atmosphere. Killing of muscle larvae by these cells in immune serum was observed. Results In immune serum, mast cells of infected rats and normal rats both killed muscle larvae. The number of muscle larvae killed was 31.5 and 17.5 （Х^2 = 13. 146,P〈0.01） ; Х^2=28. 543,P〈0.01）, respectively. Conclusion IgE antibodies enhanced the action of mast cells as part of ADCC that killed muscle larvae in rats with trichinosis.
出处
《中国病原生物学杂志》
CSCD
2011年第4期280-282,285,共4页
Journal of Pathogen Biology
