摘要
以水苏根茎为材料,进行了愈伤组织的诱导和分化、试管苗的生根、移栽和定植的研究,建立起水苏无性系技术.结果证明：MS＋BA 0.5 mg/L＋2,4-D 1.0~2.0 mg/L是愈伤组织诱导培养的理想培养基;MS＋BA 0.5 mg/L＋2,4-D1.5 mg/L是愈伤组织继代培养的理想培养基;MS＋BA 0.5 mg/L＋NAA 0.5 mg/L是愈伤组织分化的理想培养基;White＋NAA0.1 mg/L＋IAA0.4 mg/L是不定芽生根和试管苗生根继代增殖培养的理想培养基;定植的试管苗保持了野生水苏的所有生物学性状,且长势旺盛.
In order to protect wild resources and realize artificial cultivation,the rhizome of Stachys japonic were used as material to do the research on callus induction and differentiation,rooting and transplanting of tube seed-lings,finally establish the clone of Stachys japonic.The results show that the optimum medium for callus induction,subculture and differentiation is MS+BA 0.5 mg /L+2,4-D 1.0~2.0 mg /L,MS+BA 0.5 mg /L+2,4-D 1.5 mg /L and MS+BA 0.5 mg /L+NAA 0.5 mg /L respectively.White+NAA 0.1 mg /L+IAA 0.4 mg /L is the optimum medium for adventitious buds and tube seedlings rooting and subculture.Colonization of plantlets is maintained for all biological traits which wild plant has and grows vigorously.
出处
《河南科学》
2011年第5期542-545,共4页
Henan Science
基金
辽宁省高等教育教学改革资助项目(20090304)
辽宁师范大学教学改革资助项目(LSJG:20090108)
