摘要
目的:观察中医益气法对SMMC-7721和Hepa1-6肝癌细胞的体外抗癌作用;从细胞周期揭示扶正抗癌的作用机制;为采用中西结合治疗肝癌提供理论与实验依据,为肝癌的防治开辟新的研究领域。方法:运用细胞培养技术,采用现代药理学方法,MTT法从细胞水平评价益气法对肝癌细胞增殖的抑制作用。并采用流式细胞术(FCM)检测细胞周期变化,从细胞周期角度观察中医扶正治法对肝癌细胞周期的影响。结果:体外研究提示益气法对肝癌细胞具有不同程度的抑制作用,且这种抑制作用在一定范围内呈浓度依赖性,与空白组比较在50mg/mL浓度以上有统计学意义(P<0.05),益气各浓度组对两种不同的肝癌细胞有不同程度的G2/M期阻滞作用。结论:益气法在体外对肝癌细胞有抑制作用,益气法有G2/M阻滞作用。
Objective：This project is to study the anti - cancer effect in vitro of Yiqi Treatment on SMMC -7721 and Hepal - 6 liver cancer cells, thus revealing the anti - cancer mechanism of the treatment in adjusting and controlling cancer cell cycle, and supplying theoretical and experimental evidence for Fuzheng Treatment through Yiqi Treatment and the combination of Chinese and Western medicine. Methods ： Cell culture technology, modem pharmacological method and MTT method are applied to evaluate Yiqi Treatment on the proliferation of liver cancer cell. In addition ,flow cytometry （ FCM ） is used to examine cell cycle change, by which, to observe the effect of Yiqi Treatment on liver cancer cell cycle. Results：The research in vitro showed that （1） Yiqi Treatment inhibited the proliferation of liver cancer cell to some extent, and this inhibition showed a concentration dependence within a certain range and statistical significance（ P 〈 0. 05 ） compared with the control group （concentration ≥50mg/mL）. （2） Yiqi Treatment could block the cell cycle at G2/M phase. Conclusion ：The above results suggested that Fuzheng treatment showed inhibitory effect on the hepatoma in vitro. Yiqi Treatment could block the cell cycle at G2/M phase.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第5期1005-1008,共4页
Liaoning Journal of Traditional Chinese Medicine
关键词
扶正治法
益气法
肝癌
细胞周期
Fuzheng treatment
Yiqi Treatment
hepatoma carcinoma
cell cycle
