摘要
为探讨补骨脂素加锌对体外培养新生大鼠颅骨成骨细胞增殖、分化作用的影响及其量效关系,用改良的组织块培养法分离培养新生大鼠颅骨成骨细胞,在成骨细胞体系中以不同浓度加入补骨脂素与锌,MTT法检测加药后不同时间成骨细胞的增殖情况;用对硝基苯二钠基质动力学法(PNPP)测定细胞内碱性磷酸酶的活性;用放射免疫法(RIA)测定细胞外骨钙素(BGP)的含量,用改良的Lowry法测蛋白含量。补骨脂素加锌较单独应用补骨脂素或硫酸锌在48 h和72 h时促体外大鼠成骨细胞增殖的作用更加明显;在24、48 h和72 h时能提高成骨细胞碱性磷酸酶(ALP)活性,其中在48 h时的效果更为显著;在24 h和72 h时能提高细胞外液中的骨钙素含量;补骨脂素浓度为1×10-9mol/L和锌浓度为1×10-5mol/L两者联合用药较为合适。与单独应用补骨脂素或者锌相比,补骨脂素与锌联合应用能够协同增效,对体外培养的大鼠成骨细胞的增殖与分化作用更加显著。
To study the effects of psoralen plus zinc on proliferation and differentiation of cultured osteoblasts in vitro.MTT,PNPP and RIA were used to determinate the proliferation,the activity of ALP in osteoblasts and the level of BGP secreted from osteoblasts.Compared with the simple application of psoralen or zinc sulfate,psoralen plus zinc promoted the proliferation of rat osteoblast cells more distinctly after 48 h and 72 h incubation.The activity of alkaline phosphate Activity（ALP） was stimulated by psoralea and zine after 24 h and 48 h and 72 h incubation.The osteocalcin level in the medium was increased by psoralea and zine after 24 h and 72 h incubation.Psoralen plus zinc has the effects of stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第2期361-364,共4页
Natural Product Research and Development
基金
河北省自然科学基金资助项目(C2009001072)
河北省科学技术研究与发展计划项目(河北省科技支撑计划项目09276418D-14)
河北省卫生厅资助项目(04108)
