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T 细胞耐受的诱导及其机理研究 被引量:4

Study on the induction, property and mechanism of T cell anergy
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摘要 目的 阐明抗原特异性 T 细胞无能的诱导条件、无能细胞的特性及其耐受的机理。方法 抗 B71 单抗与 Cs A 联用诱导抗原特异性 T 细胞无能, 通过3 H Td R 掺入法测定 T 细胞增殖和 M L R, 利用 R T P C R 检测细胞因子基因表达。结果 耐受 T 细胞与异体淋巴细胞比例为0 .01∶1时, 可显著抑制 M L R, 转染 B71 分子的 M D A453 和3 A O 能协同刺激 C D3 诱导的 T 细胞增殖,不表达 B7 分子的 M D A453 和3 A O 无此作用。 P H A、 C D3 单抗、 P M A+ A23187 可以逆转本试验所用诱导方法所致的 T 细胞的耐受状态。无能 T 细胞 I L2 和 I F Nγm R N A 不表达, 而 I L4 和 I L10 m R N A可表达。无能 T 细胞活化后, I L2 和 I F Nγm R N A 能够表达。结论 抗原特异性 T 细胞耐受是可以人为诱导的, 无能 T 细胞细胞因子基因格局向 T H2 细胞偏离。 Study on the induction, property and mechanism of T cell anergy$$$$ FAN Zusen, MA Baoli, ZHANG Hanming. Shanghai Institute of Immunology, Shanghai Second Medical University, Shanghai 200025 【 Abstract 】 Objective To study the induction and mechanisms of T cell anergy. Methods Anergic T cells were established by combined induction of B71 McAb and CsA in vitro. T cell proliferation and MLR were detected via 3HTdR incorporation, and cytokine gene expression was analyzed with RTPCR. Results Anergic T cells significantly inhibited MLR at a ratio 0.01∶1 of anergic T cell and allolymphocyte. Anergic T cell downregulated B cell proliferation and antibody secretion. MDA453 and 3AO transfected with B71 cDNA could costimulate T cell proliferation. By contrast, MDA453 and 3AO without B7 didn't play the same role. PHA, CD3 McAb and PMA+A23187 could reverse the state of unresponsiveness of anergic T cells. Anergic T cells couldn't express IL2 and IFNγ mRNA, but could express IL4 and IL10 mRNA. Anergic T cells activated by stimuli could express IL2 and IFNγ mRNA. Conclusion Antigenspecific T cell anergy can be induced in an artificial way. Their cytokine gene profile deviate to TH2like type. 【 Subject words 】 B7/CD28 T cell anergy CsA Cytokine gene
出处 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 1999年第5期387-390,共4页 Chinese Journal of Microbiology and Immunology
基金 上海市科技发展基金
关键词 B7/CD28 T细胞无能 CSA 细胞因子基因 B7/CD28 T cell anergy CsA Cytokine gene
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