摘要
目的 检测肝癌中 Ｅｃａｄｈｅｒｉｎ 的突变情况，探讨 Ｅｃａｄｈｅｒｉｎ 与肝癌发生和发展的关系。方法 采用 Ｐ Ｃ Ｒ Ｓ Ｓ Ｃ Ｐ方法，分析了 １２ 例肝癌旁组织、５ 例肝癌栓组织和 ２２ 例肝癌组织 Ｄ Ｎ Ａ 样品中 Ｅｃａｄｈｅｒｉｎ 基因第 ６、９ 和 １０ 外显子 Ｄ Ｎ Ａ 单链构象多态性。结果 １２ 例肝癌旁组织和 ４ 例分化较好的肝癌组织中， Ｅｃａｄｈｅｒｉｎ 基因第 ６、９ 和 １０ 外显子均未见有异常。在 １８ 例恶性程度较高的肝癌组织中发现 ４ 例突变，其中 ２ 例突变发生在第 ９ 外显子，２ 例突变发生在第 ６外显子。５ 例肝癌栓组织中检出 ２ 例突变（占 ４０％ ），其中 １例突变在第 ９ 外显子，另１ 例突变发生在第 １０ 外显子。结论 Ｅｃａｄｈｅｒｉｎ 基因随肝癌的恶化，特别随转移潜能的获得，而发生突变频率的增加，表明它可能与肝癌的发展和转移相关。
Objective The purpose of this paper was to investigate the aberration of the E cadherin in human hepatocellular carcinomas, and to provide evidence for a better understanding of the development of human hepatocellular carcinoma(HCC).Methods Using the single strand conformation polymorphism analysis with polymerase chain reaction,aberration of the E cadherin exon 6,9 and 10 were examined in 22 HCC (including 4 well differentiated and 18 poorly differentiated HCCs),12 adjacent tissues of HCC and 5 HCC emboli.Results Structural abnormalities of E cadherin gene were observed in 4 of 18 poorly differentiated HCCs,(2 with an abnormal exon 9,2 with an aberrant exon 6).Two of 5 HCC emboli carried the mutation,(one with an abnormal exon 9 and the other with a mutation of exon 10).However,in 12 adjacent tissues of HCCs and 4 well differentiated HCCs,structural abnormalities of E cadherin exon 6,9 and 10 were not found.Conclusions The results suggest the involvement of E cadherin gene in development and metastasis of HCC.$$$$
出处
《肿瘤》
CAS
CSCD
北大核心
1999年第5期263-266,共4页
Tumor
