摘要
目的:研究石杉碱甲对老年大鼠海马、皮层和血清的脂质过氧化和超氧化物歧化酶的作用。方法:硫代巴比妥酸法测定组织中MDA水平;黄嘌呤氧化酶法测定组织中超氧化物歧化酶的活性。结果:雄性老年大鼠海马、皮层和血清中MDA水平和Mn-SOD活性明显高于成年大鼠。石杉碱甲明显降低雄性老年大鼠海马、皮层和血清中MDA水平和SOD活性,而对成年大鼠的这两项指标无明显影响。结论:石杉碱甲能明显改善衰老导致的自由基系统的异常变化,这种神经保护作用可能有益于AD的治疗。
AIM: To study the effects of huperzine A on lipid peroxidation and superoxide dismutase ( SOD) in hippocampus, cerebral cortex, and serum of aged rats. METHODS: The level of lipid peroxidation was determined by thiobarbituric acid reactive substance method and represented as the concentration of malondialdehyde (MDA) in wet tissue. The activity of SOD was determined by xanthine-xanthine oxidase method and represented as the nitrite unit per gram protein in wet tissue. RESULTS: The levels of MDA and the manganese-SOD (Mn-SOD) activities in hippocampus, cerebral cortex, and serum of aged male rats were 2.3 - 2.8 times and 1.8 - 2.8 times, respectively, higher than those of adult male rats. Huperzine A (0.05 mg/kg, ig) lowered markedly the levels of MDA and the activities of Mn-SOD in aged male rats following 7 - 14 d consecutive administrations. The MDA levels in hippocampus, cerebral cortex, and serum decreased 44.7 - 52.8 % (7 d) and 52.6- 54.7 % (14 d). The Mn-SOD activities in hippocampus, cerebral cortex, and serum lowered 25.0 -57.6 % (1 d) and 56.0-74.2 % (14 d). In adult rats, no marked change was found after 7 - 14 d consecutive administrations of huperzine A at a dose of 0.05 mg/kg. CONCLUSION: Huperzine A improved the abnormal free radicals in aged rats.
出处
《中国药理学报》
CSCD
1999年第9期824-828,共5页
Acta Pharmacologica Sinica
关键词
自由基
衰老
脂质过氧化作用
丙二醛
石杉碱甲
free radicals
aging
lipid peroxidation
malondialdehyde
superoxide dismutase
hippocampus
cerebral cortex
huperzine A
