摘要
目的观察小分子蛋白多糖decorin（DCN）局部注射对兔耳增生性瘢痕的抑制作用。方法新西兰大白兔8只，建立增生性瘢痕动物模型，随机分成两组。两组均于术后第20天、第30天行瘢痕内注射，实验组（A组）注射decorin，对照组（B组）注射生理盐水。末次注射后20d取材，观察瘢痕增生；苏木素．伊红（HE）染色比较瘢痕增生指数，免疫组织化学检测瘢痕组织中I型胶原蛋白表达；逆转录-聚合酶链反应（1IT—PCR）检测I型前胶原mRNA表达。结果A组上皮角化良好，瘢痕质地较软，色泽与周围正常皮肤接近。A组瘢痕增生指数为2．8970±0．2066（B组3．5210±0．1960）；I型胶原蛋白表达值为0．22120±0．02706（B组0．33690±0．03074）；I型前胶原mRNA表达值为0．37250±0．02678（B组0．64560±0．02994），与B组比较均降低，差异有统计学意义（P〈0．05）。结论小分子蛋白多糖deeorin局部注射可以抑制早期兔耳腹增生性瘢痕，减轻瘢痕的增生程度。
Objective To observe the inhibitory effect of decorin on hypertrophic scar in rabbit cars. Methods Full-thickness excisional wounds, 8 mm in diameter, were made over the ventral side of ears in 8 adult New Zealand white rabbits, and 48 hypertrophic scars in total. Then the rabbits were divided into two groups： group A, experimental group （decorin, 10 mg/L） ; group B, control group （physiological saline）. At the 20th and 30th day after operation, the hypertrophic scars were injected with decorin （group A） and saline （group B ） intralesionally. Samples were harvested 20 days after the last injection （ 50th day） for measurement of scar elevation index （SEI）. The expression of type I collagen protein and type I pro-collagen mRNA was assessed by immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR） respectively. Results Compared with group B, the scars appeared to be flatter, softer, and lighter in color in group A. The SEI in group A and group B was 2. 8970 ±0. 2066 and 3. 5210 ± 0. 1960 respectively. The expression of type I collagen protein and type I pro-collagen mRNA in group A was 0. 221 20 ±0. 027 06 and 0. 372 50 ±0. 026 78 respectively, and that in group B was 0. 336 90 ±0. 030 74 and 0. 645 60 ± 0. 029 94 respectively （ all P 〈 0. 05 ）. Conclusion Decorin can inhibit the proliferation process of hypertrophic sear in rabbit ears and remarkably decrease the degree of fibrosis in the scar.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第5期691-693,共3页
Chinese Journal of Experimental Surgery
关键词
增生性瘢痕
蛋白多糖
Hypertrophic scar
Proteoglycan
