摘要
目的:筛选出促进地黄生长的内生真菌,并初步研究其促生作用。方法:通过4株分离自地黄的内生真菌与地黄无菌苗及地黄盆栽苗的共生实验,以苗重,叶绿素含量和根冠比为指标筛选出促进地黄生长的内生真菌,通过PC比色法测定真菌分泌IAA的能力并研究内生真菌的促生作用。结果:通过共生实验,发现4株真菌中,Ceratobasidium属内生真菌-菌Ⅳ显著促进盆栽地黄生长(P<0.05),与该菌共生的地黄无菌苗平均增长量是对照组正常生长地黄的1.25倍,并显著提高叶片中的叶绿素含量(P<0.01)。通过salkowsk i比色法表明Ceratobasidium属内生真菌-菌Ⅳ分泌IAA,IAA浓度的峰值约可达到5.712 mg.L-1。结论:Ceratobasidium属内生真菌-菌Ⅳ促进地黄生长。
In previous studies,four endophytic fungi were isolated from different swollen roots of Rehmannia glutinosa.It′s thought that Ceratobasidium sp.,one of the discovered endophytic fingi,was a major promoter for the growth of the roots.In this study,symbiotic experiments were performed to measure the effects of different endophytic fingi cultivated with R.glutinosa.The results showed that the R.glutinosa had significant increases in the size of roots and amount of chlorophyll cultivated with Ceratobasidium sp.And it was tested that indoleacetic acid secreted by Ceratobasidium sp.maybe the effective factor for the promotion of the growth.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第9期1137-1140,共4页
China Journal of Chinese Materia Medica
基金
国家"十一五"科技支撑计划子课题(2006BAI09B01-10)
国家中医药管理局行业专项(201107011)
关键词
地黄
内生真菌
促生作用
IAA
Rehmannia glutinosa endophytic fingi； promoting effects of growth； indoleacetic acid；
