摘要
根据cry1I类基因的全长序列设计引物,以苏云金芽胞杆菌(Bacillus thuringiensis,Bt)菌株BtMX2的总DNA为模板扩增出片段长为2.1kb的cry1I的全长基因,插入大肠杆菌(Escherichia coli)表达载体pEB,转化大肠杆菌Rosetta菌株,诱导表达出81ku的蛋白,编码的蛋白由720个氨基酸组成,该蛋白等电点为6.09,为弱酸性蛋白。与已知cry1Ia9基因的氨基酸一致性为99%,相差2个氨基酸。该基因的登录号为GU989199,已被国际基因命名委员会正式命名为cry1Ia17。
A full-length cry1Ia gene fragment,which obtained by PCR amplification with a pair of primers designed according to cry1I-type gene sequences and DNA from Bacillus thuringiensis BtMX2 as template,was introduced into expression vector pEB and transformed into Escherichia coli Rosetta,the predicted MW was 81 ku.Molecular weight of the induced express product was 81 ku.The encoded protein was composed of 720 amino acid residues,the isoelectric point of the protein was 6.09,and it was the weak acid protein.The amino acid sequence of cry1Ia17 was with an identity of 99% to cry1Ia9,the difference was two amino acids.The gene accession number was GU989199 and was designated as cry1Ia17 by International Nomenclature Committee of Bt.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第4期93-97,共5页
Journal of Northeast Agricultural University
基金
转基因生物新品种培育重大专项(2009zx08009-031B)
植物病虫害生物学国家重点实验室2010年度开放基金资助课题(skl20100p13)
