摘要
目的探讨确认试验在乙型肝炎表面抗原（HBsAg）弱阳性标本中的临床应用。方法对酶联免疫吸附试验（ELISA）检测的HBsAg结果在0.875~2.857的血清标本进行抗体中和确认试验,并进行微粒子酶免疫发光（MEIA）试验,对比各试验结果间的差异。结果 ELISA检测阳性75份阳性率83.3%,抗体中和试验确认试验阳性76份阳性率84.4%,两项试验检测结果间差异有统计学意义（P〈0.01）。结论 ELISA试验检测HBsAg结果为弱阳性的标本,应进行确认试验,排除假阳性,保证样本结果的真实性,对减少医疗纠纷、防止医源性感染具有重要意义。
OBJECTIVE To study the clinical application of the confirmatory test for weak positive hepatitis B surface antigen（HBsAg）.METHODS A total of 90 HBsAg serum samples,which were screened by enzyme-linked immunosorbent assay（ELISA） and their S/CO were 0.875-2.857,were retested by antibody neutralization confirmatory test and microparticle enzyme immunoassay（MEIA）.The differences between the results of these tests were compared.RESULTS The positive results of ELISA and antibody neutralization confirmatory test were 75（83.3%） and 76（84.4%） respectively.The difference between the results of the two tests had statistical significance（P0.01）.CONCLUSION The samples with weak positive results detected by ELISA method should have the confirmatory test.It ensures the real results of samples.It has important significance to decrease of medical dissatisfaction and prevent nosocomial infection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第10期2152-2153,共2页
Chinese Journal of Nosocomiology
关键词
抗体中和确认试验
酶联免疫吸附试验
乙型肝炎表面抗原
弱阳性
Antibody neutralization confirmatory test
Enzyme-linked immunosorbent assay
Hepatitis B surface antigen
Weak positive
