摘要
AIM:Coptidis Rhizoma(CR),the dried rhizomes of Asian herbs(including Coptis chinensis Franch),has been used to treat diabetes mellitus for thousands of years.We explored the possibility that CR acts directly on skeletal muscle,the major organ responsible for glucose homeostasis,and activates 5'-AMP-activated protein kinase(AMPK),a signaling intermediary leading to metabolic enhancement of skeletal muscle.METHODS:Isolated rat epitrochlearis and soleus muscles were incubated in a buffer containing a CR water extract(CE),and activation of AMPK and related events were examined.RESULTS:In response to CE treat-ment,phosphorylation of Thr172 at the catalyticαsubunit of AMPK,an essential step for full kinase activation,increased in both mus-cles.Phosphorylation of Ser79 of acetyl CoA carboxylase(ACC),an endogenous substrate of AMPK,increased concomitantly.Analysis of isoform-specific AMPK activity revealed that CE activated both the α1 and α2 isoforms of the catalytic subunit.Importantly,the maximal effect of CE on AMPK phosphorylation was significantly greater than that of berberine(BBR),indicating that the action of CE is not totally ascribed to BBR.CONCLUSION:We propose that CE is an acute activator of AMPK in both fast-and slow-twitch skeletal muscles.
AIM：Coptidis Rhizoma（CR）,the dried rhizomes of Asian herbs（including Coptis chinensis Franch）,has been used to treat diabetes mellitus for thousands of years.We explored the possibility that CR acts directly on skeletal muscle,the major organ responsible for glucose homeostasis,and activates 5＇-AMP-activated protein kinase（AMPK）,a signaling intermediary leading to metabolic enhancement of skeletal muscle.METHODS：Isolated rat epitrochlearis and soleus muscles were incubated in a buffer containing a CR water extract（CE）,and activation of AMPK and related events were examined.RESULTS：In response to CE treat-ment,phosphorylation of Thr^172 at the catalyticαsubunit of AMPK,an essential step for full kinase activation,increased in both mus-cles.Phosphorylation of Ser^79 of acetyl CoA carboxylase（ACC）,an endogenous substrate of AMPK,increased concomitantly.Analysis of isoform-specific AMPK activity revealed that CE activated both the α1 and α2 isoforms of the catalytic subunit.Importantly,the maximal effect of CE on AMPK phosphorylation was significantly greater than that of berberine（BBR）,indicating that the action of CE is not totally ascribed to BBR.CONCLUSION：We propose that CE is an acute activator of AMPK in both fast-and slow-twitch skeletal muscles.
出处
《中国天然药物》
SCIE
CAS
CSCD
北大核心
2011年第3期215-221,共7页
基金
supported by the research grants from the Japan Society for the Promotion of Science (No.20500576)
Japan Vascular Disease Research Foundation
