摘要
目的观察三维回转模拟失重对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hP-DLFs)形态和功能的影响。方法回转组hPDLF细胞运用三维回转器(TDC)分别培养48 h,72 h和96 h,对照组常规培养。利用HE染色、细胞计数法、MTT活性检测、流式细胞术、Hoechst 33258核染色及免疫荧光分析的方法,比较两组细胞间形态、增殖、周期、凋亡及细胞骨架等生物学性能的差异。结果与各自对照组相比,hPDLF细胞三维回转培养48 h后,细胞核面积较对照组缩小(P<0.01),微丝结构略显模糊,但细胞增殖和凋亡无明显变化;回转72 h后,细胞活力显著增强(P<0.01),但细胞形态与凋亡无明显变化;回转96 h后,hPDLF细胞增殖能力较对照组显著增强(P<0.01),细胞活力明显升高(P<0.01),S期所占周期比例明显增加(P<0.05),细胞形态与凋亡与对照组比较仍无明显差异。结论短期(48 h)暴露于三维回转培养条件使hPDLF细胞骨架结构和胞核形态发生变化,但随着刺激时间的延长(72 h后),细胞内部恢复正常,并表现出细胞活力及增殖能力增强的趋势。
Objective To investigate the biological characteristics of human periodontal ligament fibroblasts（hPDLFs） under simulated weightlessness environment by using three dimensional clinostat（TDC） Methods Clinostat group was cultured in TDC for 48 h,72 h and 96 h;control group was cultured conventionally.Then,HE staining,cell counting,MTT assay,flow cytometry and immunofluorescence method were used in both groups to analyze their differences in morphology,proliferation,cell cycle,apoptosis and cytoskeleton.Results Compared with the control,the nucleic area of hPDLFs decreased（P0.01） and the cytoplasmic microfilament arrangement disorganized with low-grade,however,the proliferation and apoptosis were not significantly changed after exposure to simulated weightlessness for 48 h.The viability of hPDLFs were significantly enhanced（P0.01）,but the morphology and apoptosis of hPDLFs did not change after exposure to simulated weightlessness for 72 h and 96 h.In addition,the cell cycle assay demonstrated that an increase in the percentage of hPDLFs arrested in S phase when compared with the control（P0.05） after 96 h exposure.Conclusion There are changes in intracellular cytoskeleton and the nucleic morphology of hPDLFs after exposure to simulated weightlessness for short time（48 h）.However,after 72 h the morphology and structure of hPDLFs can recover gradually,as well as the viability and prolifertion tend to enhance.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2011年第2期96-99,共4页
Space Medicine & Medical Engineering
关键词
失重模拟
三维回转器
人牙周膜成纤维细胞
增殖
凋亡
weightlessness simulation
three-dimensional clinostat
human periodontal ligament fibroblasts
proliferation
apoptosis
