摘要
目的研究刺五加对睡眠剥夺大鼠学习记忆能力及海马长时程增强的影响。方法采用小平台法建立快速眼动睡眠(REMS)剥夺大鼠模型,利用行为学及神经电生理学方法,观察刺五加对其学习记忆能力及海马突触长时程增强(LTP)的影响,探讨刺五加对睡眠剥夺造成的学习记忆损害的保护作用及作用机制。结果与大平台对照组比较,睡眠剥夺组错误次数增多、认知率降低,且差异显著(P<0.01),但寻找时间缩短(P<0.05);在刺五加干预下,错误次数明显减少、认知率升高(P<0.01),寻找时间缩短(P<0.01)。睡眠剥夺组大鼠LTP明显受到抑制,高频刺激(HFS)引起的群峰电位幅值及兴奋性突触后电位斜率的增大明显低于大平台对照组(P<0.01),各剂量给药组对睡眠剥夺引起的LTP抑制现象均有明显恢复作用(P<0.01)。结论睡眠剥夺能引起动物学习记忆能力的降低,刺五加可对抗此作用,其机制可能与改善神经突触可塑性有关。
Objectve： Study the effect of acanthopanx on learning and memory and LTP in hippocampus of sleep deprived rats.Methods： The model of rapid eye movement sleep（REMS） deprivation in rats was established by using a small platform,ethology and nerve electrophysiology method were performed to observe that the effect of acanthopanax on learning and memory and LTP in hippocampus of rats under rapid eye movement（REM） sleep deprived conditions.We discuss the protective effect and mechanism of acanthopanax from learning and memory damage which was induced by sleep deprivation.Results： Compared with the large platform control group,the number of errors of sleep deprivation group increased,the rate of cognitive reduced,and the difference is significant（P〈0.01）,but the searching time shortened（P〈0.05）;In the intervention of acanthopanax,the number of errors decreased significantly,the rates of cognitive ascended（P〈0.01）,the searching time decreased（P〈0.01）.LTP of sleep deprivation in rats was inhibited significantly,population spike amplify（PSA） and EPSP slope introduced by high frequency stimulation（HFS） were lower than the large platform control group significantly（P〈0.01）,all treatment groups had significant recovery effect on LTP suppression induced by sleep deprivation（p〈0.01）.Conclusion： Acanthopanax can against the reduction of animal learning and memory that induced by sleep deprivation,the mechanism may be related to improvement of the synaptic plasticity.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2011年第1期63-66,共4页
Pharmacology and Clinics of Chinese Materia Medica
关键词
刺五加
睡眠剥夺
学习记忆
长时程增强
Acanthopanax（刺五加）； sleep deprivation； learning and memory； LTP；
