摘要
目的:探讨p53和c-erbB-2的表达阳性率和强度与胃癌浸润深度的关系。方法:收集139例胃癌手术切除标本,包括黏膜内癌23例、浸润至黏膜下层31例、浸润至肌层47例、浸润至浆膜或浆膜外38例,切片采用免疫组化S-P法染色,观察p53和c-erbB-2的表达阳性率和强度。结果:不同浸润深度的胃癌中p53的阳性率比较,黏膜内癌组和浸润至黏膜下层组与浸润至浆膜层或浆膜外组存在显著差异。而各组c-erbB-2的表达阳性率比较也均存在显著差异(P<0.01)。p53的表达强度在不同的浸润深度无显著差别(P>0.05),而c-erbB-2差别明显,在胃癌浸润至浆膜或浆膜外时,其表达强度最高(P<0.01)。c-erbB-2表达阳性率与p53存在相关性(P<0.05),而表达强度则无明显相关性(P>0.05)。p53和c-erbB-2的不同表达强度中,p53Ⅲ、c-erbB-2Ⅰ、c-erbB-2Ⅱ和c-erbB-2Ⅲ对胃癌浸润深度均有影响(P分别为0.001、0.010、0.000、0.000)。结论:p53和c-erbB-2在胃癌中的表达不是同步的,p53突变有利于c-erbB-2的表达,但c-erbB-2的表达强度与p53无关。抑癌基因p53突变可能与胃癌的起源有关;胃癌的浸润主要与癌基因c-erbB-2的激活有关。
Objective：To explore the positive rate and expression levels of p53 and c-erbB-2 in gastric cancer and their relationship with tumor invasion.Methods：SP immunohistochemistry was used to detect the positive rates and expression of p53 and c-erbB-2 expressed in 139 gastric cancer with different invasive depth of lamina propria（T1a）,submucosa（T1b）,muscularis propria（T2） and suserosa（T3）.Results：The positive rates of p53 in T1a and T1b tumors were lower than T3 tumors significantly（P 〈 0.05）,but the expression levels of p53 hadn′s significant difference among tumors with different invasive depth（P 〈 0.05）.The positive rates and expression levels of c-erbB-2 augmented with increased invasive depth of tumor（P 〈 0.01）.The positive rates of c-erbB-2 were related with p53（P 〈 0.05）,but the expression levels of c-erbB-2 and p53 hadn′t significant relationship（P 〉 0.05）.Ordinal logistic regression analysis showed that both intensity expression of p53 and low,medium,high degree of c-erbB-2 expression affected the invasive depth of gastric cancer significantly（P=0.001,P=0.010,P=0.000,P=0.000,respectively）.Conclusion：The expression of p53 and c-erbB-2 in gastric cancer are not synchronous and p53 mutation contributes to c-erbB-2 expression,but the expression level of c-erbB-2 is not related with p53.The mutation of anti-oncogene p53 maybe related to the origin of gastric cancer,while the invasion of gastric cancer is mostly relate to activation of oncogene c-erbB-2.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第3期422-426,共5页
Journal of Nanjing Medical University(Natural Sciences)
