摘要
大豆基因组测序与注释为重要基因的发现和基因功能、进化和发生的研究提供了坚实基础。Nramp蛋白是古老的膜整合转运蛋白家族,在重金属抗性中起着重要的作用。本研究利用BLAST从大豆基因组搜索出8个编码Nramp蛋白的基因,GmNramp氨基酸序列非常保守,具有典型的Nramp结构域,预测的跨膜结构域10-11个。根据结构的差异,GmNramp蛋白分为两个亚家族。大豆GmNramp基因家族的扩张主要是由于染色体复制引起的,并且GmNramp在不同的组织或器官中可被转录,表明GmNramp在大豆生长发育过程具有一定的功能。研究结果为进一步分离大豆GmNramp基因奠定了基础。
Soybean genome sequencing and annotation provides a solid foundation for identification of important genes and studies of gene function, evolution and genesis. Nramp proteins is an ancient family of membrane transport proteins, and play an important role in resistance to heavy met al. In this study, eight genes encoding Nramp proteins were identified from soybean genome, using BLAST. GmNramp amino acid sequences were very conservative, with a typical Nramp domain and the number of predicted transmembrane of GmNramps varied from 10 to 11. GmNramp proteins were thus divided into two subfamilies, according to structural difference. The expansion of GmNramp gene family was mainly caused by the chromosome duplication, and GmNramp could be transcribed in different tissues or organs, indicating that GmNramp have certain functions for growth and development of soybean. The results provided for basis for further cloning of soybean GmNramp.
出处
《分子植物育种》
CAS
CSCD
2011年第2期245-250,共6页
Molecular Plant Breeding
基金
福建省科技厅青年人才项目(2006F3026)
农业部公益性行业(农业)科研专项(200903002)
福建省公益类科研院所基本科研专项(2009R10027-3)共同资助
