摘要
目的观察消癌平对化疗药物5-氟尿嘧啶(5-Fu)的最佳增效时间和最佳增效浓度,并探讨其发挥化疗增效作用的机制。方法采用噻唑蓝(MTT)比色法观察不同浓度消癌平联合5-Fu对Hepa1-6肝癌细胞的生长抑制作用,消癌平作用浓度分别设10、305、0 mg/mL,作用时间分别设5-Fu作用前0、8、16、24 h和作用后8、16、24 h,采用细胞划痕愈合实验测定细胞扩散运动能力。结果不同浓度消癌平联合5-Fu化疗组细胞生长抑制率均明显高于5-Fu单药组(P均<0.05),且消癌平的增效作用随其剂量的增加而增加(P<0.05),50mg/mL消癌平于5-Fu作用前8 h加入增效作用最佳(P<0.01),消癌平8 h-5-Fu给药组较肝癌细胞组、5-Fu单药组能显著减弱细胞移行运动能力。结论消癌平以50 mg/mL的浓度于5-Fu作用前8 h加入化疗增效作用最佳,消癌平主要通过减弱细胞移行运动能力发挥增加5-Fu的化疗作用。
Objective To observe the best enhancement time and concentration of Xiaoaiping on chemotherapeutic response of 5-fluorouracil（5-Fu） and to explore the mechanism of chemosensitization.Methods MTT method was used to test the growth inhibitory rate of hepatocellular carcinoma cell Hepa1-6 acted by Xiaoaiping and5-Fu.TheconcentrationofXiaoaiping was 0,30,50 mg/mL and the time point was 0,8,16,24 h before 5-Fu,and 8,16,24 h after 5-Fu.Monolayer wound healing assay was used to detect Hepa1-6 cell motility.Results The inhibitory rate of cell growth in Xiaoaiping combination groups was significantly higher than that in 5-Fu alone group（P0.05）,and the enhancement increased with Xiaoaiping dosage augmented.50 mg/mL Xiaoaiing could exert the best enhancement on chemotherapeutic response of 5-Fu when it was added 8 h before 5-Fu（P0.01）.Xiaoaiping 8 h 5-Fu group can weaken the cell migration ability significantly compared with hepatocellular carcinoma group and 5-Fu alone group.Conclusion Xiaoaiping could exert the best enhancement with 50 mg/mL when added 8 h before 5-Fu.
关键词
癌
肝细胞
消癌平
氟尿嘧啶
肿瘤治疗方案
Carcinoma
hepatocellular
Xiaoaiping
Fluorouracil
Antineoplastic protocols
