摘要
目的观察17β-雌二醇(E2)对巨噬细胞胞浆游离钙离子浓度([Ca2+]i)的影响及可能的作用途径。方法小鼠腹腔巨噬细胞用F lou-3/AM负载,用激光共聚焦显微镜检测,[Ca2+]i以细胞内荧光强度表示。结果 100 nmol/L的17β-雌二醇能使小鼠腹腔巨噬细胞的[Ca2+]i明显提高(提高率39.8%)。巨噬细胞在无钙的条件下,加入E2后[Ca2+]i升高10.6%,在补充入Ca2+后,[Ca2+]i更有明显升高。用维拉帕米、肝素和普鲁卡因预先处理后,E2诱导的[Ca2+]i升高分别被抑制了52.8%,16.6%和36.7%。结论 E2诱导的腹腔巨噬细胞胞浆游离钙的升高是外钙内流和内钙释放共同作用的结果,并且这种作用与L-型钙通道、IP3受体和ryanod ine受体关系密切。
Objective It is to observe the influence of 17β-estradiol（E2） on intracellular Ca2＋ concentration（i） in macrophages and the possible action path.Methods Macrophages were loaded with Flou-3/AM and fluorescent intensity which represented i was detected with laser scanning confocal microscopy（LSCM）.Results i heightened 39.8% in macrophages after treated with E2（100 nmol/L）.E2 induced i elevation only 10.6% in noncalcium condition and i increased more obviously after supplement with CaCl2.Pretreated with verapamil,heparin and procaine,the increases of i induced by E2 were inhibited 52.8%,16.6% and 36.7% respectively.Conclusion E2 induces i heightening in macrophages is the result of Ca2＋ entry from the extracellular medium and calcium release from intracellular calcium stores.Furthermore,the action has close relation with L-type calcium channel,IP3 receptor and ryanodine receptor.
出处
《现代中西医结合杂志》
CAS
2011年第14期1705-1707,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家科技部十五攻关项目(2001BA701A29)
江苏省科技厅自然科学基金项目(BK2000034)
江苏省"青蓝工程"资助
南京中医药大学科技创新团队资助
