摘要
目的 观察腺病毒介导的肝细胞生长因子（Ad-HGF）对脂肪干细胞的感染效率以及感染后是否可形成有效的肝细胞生长因子（HGF）,确定感染强度（MOI）值.方法 利用消化分离方法和脂肪干细胞贴壁生长的特性,分离人脂肪干细胞,利用相同MOI的Ad-HGF感染脂肪干细胞,ELISA法检测HGF的表达.结果 脂肪干细胞均呈贴壁生长的成纤维细胞样形态,原代培养的细胞7～10 d即达70%～80%融合,Ad-HGF感染脂肪干细胞后HGF可在48 h高效表达.结论 提示腺病毒可有效介导HGF基因,可感染脂肪干细胞,并能够产生有效浓度的HGF.
Objective To observe the efficiency of infection of adenovirus containing hepatocyte growth factor（Ad-HGF） on adipose derived stem cells and to prove whether the valid HGF can appear after infection and the multiplicity of infection. Methods We use the digestion separation method and the attachingwall characteristic of the adipose-derived stem cells to separate the human adipose-derived stem cells. Adipose-derived stem cells were infected by the vector of adenovirus （Ad-GFP） which carries the GFP gene,and the GFP acts as the indicating gene to determine the infection efficiency of recombinant adenovirus to adipose- derived stem cells. HGF-ELISA was used to detect HGF as expression-secretion. Results The adherent cells displayed themselves as fibroblast in morphology. The primary cultured cells fusion can arrive to 70% - 80% in 7 - 10 days. The infected HGF can be highly expressed in 48hours. Conclusion Adenovirus can meditate the expression of HGF gene in adipose-derived stem cells effectively.
出处
《国际外科学杂志》
2011年第4期235-238,F0003,共5页
International Journal of Surgery
关键词
肝细胞生长因子
腺病毒载体
脂肪干细胞
Hepatocyte growth factor
Adenovirus vector
Adipose tissue-derived stromal cells
