摘要
目的观察红花对兔耳增生性瘢痕(HS)及其转化生长因子β1(TGFβ1)表达的影响。方法取27只新西兰大白兔兔耳腹侧建立HS模型,每耳2块。术后第45天开始对HS行注射治疗,每周1次,连续注射4次。设立正常皮肤组、阳性对照组、生理盐水组、低浓度红花(125 g/L)组、高浓度红花(500 g/L)组。注射完成后第2、4、6周分别测量HS厚度及硬度并切取8只兔耳HS及皮肤,采用免疫组织化学方法检测每块组织TGFβ1蛋白的表达(TGFβ1蛋白面密度),RT-PCR方法检测每块组织TGFβ1mRNA的表达。结果注射后4、6周,高浓度红花组HS厚度及硬度均较其他HS组低(P<0.05);注射后2、4、6周,高浓度红花组TGFβ1蛋白面密度值及TGFβ1mRNA的表达均较其他HS组低(P<0.05);低浓度红花组TGFβ1mRNA的表达在注射后4、6周均较阳性对照及生理盐水组低(P<0.05)。结论红花能促进兔耳HS的软化、变薄,并能抑制兔耳HS的TGFβ1的表达,尤以高浓度红花组明显。红花抑制兔耳HS的机制可能与TGFβ1的表达被抑制相关。
Objective To investigate the effect of carthamus tinctorius on hypertrophic scar（HS） of rabbit′s ears and its transforming growth factor-β1（TGFβ1） expression.Methods 27 New Zealand rabbits were chosen as experiment animals.The models of HS were established with 2 HS in each ear.On 45 d after operation,HS of a rabbit was injected with nothing,sodium chloride,carthamus tinctorius（125 g/L） and carthamus tinctorius（500 g/L） respectively.The treatment was done once a week for 4 weeks.On 2,4,6 weeks after injection,HS and skin of 8 rabbits that were chosen randomly were measured their thicknesses and hardnesses,were harvested and detected by immunohistochemical method and reverse transcription polymerase chain reaction（RT-PCR）.Results On 4,6 weeks after injection,the thicknesses and the hardnesses of group carthamus tinctorius（500 g/L） were lowest in all HS groups（P0.05）.The area density of TGFβ1 protein and expression of TGFβ1 mRNA in group carthamus tinctorius（500 g/L） were both the lowest in all HS groups on 2,4,6 weeks after injection（P0.05）.The expression of TGFβ1 mRNA in group carthamus tinctorius（125 g/L） was lower than positive control and sodium chloride groups on 4,6 weeks after injection（P0.05）.Conclusion Carthamus tinctorus could enhance HS in rabbit′s ears softening,and could inhibit the expression of TGFβ1.The effect of carthamus tinctorius（500 g/L） is more than carthamus tinctorius（125 g/L）.The mechanism inhibiting HS of rabbit′s ear is correlative possibly to the expressions inhibition of TGFβ1 by carthamus tinctorius.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第13期1294-1296,共3页
Chongqing medicine
基金
重庆市卫生局科研项目(渝中医〔2002-63〕)
重庆医科大学儿科学院资助课题
关键词
瘢痕
红花
转化生长因子Β1
cicatrix
carthamus tinctorius
transforming growth factor betal
