摘要
以新疆陆地棉主栽品种新陆早19号和新陆早23号下胚轴为外植体,通过对再生体系的研究,首次获得了胚性愈伤组织和再生植株。愈伤组织诱导以0.10 mg/L KT和0.10 mg/L 24,-D为最佳,KNO3加倍有利于胚性愈伤组织形成,铺垫滤纸有利于胚状体的成熟和发达根系的产生。用此法,实验材料在6～8个月内即可获得大量再生苗。
An efficient somatic embryo procedure was developed to regenerate plantlets from hypocotyls of cotton cultivars Xinluzao 19,Xinluzao 23 grown in Xinjiang.Callus were effectively produced on the medium with 0.10mg/L Kinetin（KT） and 0.10mg/L 2,4-Dichlorophenoxyacetic acid（2,4-D）.Double amounts of KNO3 in the medium were beneficial to the emerge of embryogenic call.Filter paper in the medium pad could promote somatic embryos grow and root system regeneration.Using this protocol,two experimental materials could get a large number of regenerated plantlets in 6 to 8 months.
出处
《种子》
CSCD
北大核心
2011年第4期1-3,7,共4页
Seed
基金
国家自然科学基金(编号:30760030)
高等学校博士学科点专项科研基金(编号:200807550001)
关键词
陆地棉
新陆早系列
体细胞胚胎发生
植株再生
cotton（Gossypium hirsutum L.）
xinluzao series
somatic embryogenes
plant regenerations
