摘要
目的：观察脑缺血半暗区脑红蛋白（neuroglobin,NGB）与细胞色素C（Cyt-C）相关性,以探讨NGB在脑缺血早期是否通过Cyt-C途径抑制Caspase-3表达。方法：大鼠随机分为假手术对照组（Sham）、缺血再灌注组（CIR）、干预组（IV）和干预对照组（IVC）。CIR组制作脑缺血再灌注模型,按断头时程不同又分为1 h、6 h、12 h、24 h、72 h 5个亚组。IV组将重组人NGB注入左侧尾壳核30 min后制作脑缺血再灌注模型,按断头时程不同又分为1 h、6 h、12 h、24 h、72 h 5个亚组,其中Sham组和IVC组于24 h断头。取脑作HE和免疫组化染色。结果：NGB在缺血再灌注1h已经升高,24 h后达高峰,然后逐步下降。Caspase-3和Cyt-C在1 h后表达增多,12-24 h增高达到高峰,然后逐步下降。干预后Cyt-C表达明显减少（P〈0.01）,Caspase-3表达也明显减少（P〈0.05）。结论：脑缺血后NGB在缺血半暗区先升高后下降,可能通过Cyt-C途径抑制Caspas-3表达而发挥脑保护作用。
Objective：To find out whether neuroglobin（NGB） could regulate caspase-3 expression through pathway of cytochrome（Cyt-C） in penumbra after cerebral ischemia reperfusion by examining the correlation of the two factors.Methods：Seventy-two male Sprague-Dawley（SD） rats were randomized into groups of sham operation（Sham）,cerebral ischemia-reperfusion（CIR）,intervention（IV） and interventional controls（IVC）.After CIR models were developed,the rats were decapitated and subgrouped chronologically into CIR of 1 h,6 h,12 h,24 h and 72 h,respectively.In IV group,CIR models were established 30 min after injection of recombinant human NGB into the left striatum,followed by subgrouping chronologically into CIR of 1 h,6 h,12 h,24 h and 72 h,respectively.The animals in both Sham and IVC were killed at 24 h.Cerebral specimens were obtained for section with HE and immunohistostaining.Results：NGB expression was found up-regulated at 1h after CIR and peaked at 24 h before it down-regulating progressively.Increased expression was seen at 1h for caspase-3 and Cyt-C,topping at 12～24 h and descendeing thereafter.After injection of recombinant human NGB into the left striatum,expression of Cyt-C and caspase-3 was seen down-graded significantly（P〈0.01,P〈0.05）.Conclusion：NGB was increased before fall in ischemic penumbra after cerebral ischemia-reperfusion,suggesting that NGB may be involved in the neuroprotection by inhibiting caspas-3 expression through pathway of Cyt-C.
出处
《皖南医学院学报》
CAS
2011年第2期87-91,共5页
Journal of Wannan Medical College
基金
院中青年科研基金项目(WK200825F)
