摘要
将奥默柯达酵母(Kodamaea ohmeri)植酸酶基因phy1构建到巴斯德毕赤表达载体pPICZαA中,转入到受体菌X-33中,成功实现了奥默柯达酵母植酸酶基因phy1的分泌表达。利用BMMY培养基进行诱导培养,甲醇诱导3 d后,重组酶产量达到最高,为6 290 U/mL,SDS-PAGE显示重组酶的大小约为62 ku。通过对表达产物的酶学性质研究,发现该重组酶具有较好的热稳定性及较广泛的pH适用范围,其最适作用温度为65℃,最适作用pH为5.0。
Kodamaea ohmeri phytase gene phy1 was inserted into the expression vector pPICZαA and expressed in Pichia pastoris X-33.After 3 d of methanol induction,the activity of recombinant phytase was up to 6 290 U/mL using BMMY medium.The SDS-PAGE indicated that the size of recombinant enzyme was nearly 62 kDa.The optimal temperature and pH of the recombinant phytase was 65 ℃ and 5.0,respectively,and the enzyme had good thermal stability and broad pH range of application.
出处
《热带作物学报》
CSCD
2011年第2期257-260,共4页
Chinese Journal of Tropical Crops
基金
海南省教育厅高等学校科学研究资助性项目(Hjkj2010-14)
海南大学科研启动基金项目(kyqd1021)
